Octanoylcarnitine

high-resolution terminology - matching measurements at high-resolution

Octanoylcarnitine

Description

Octanoylcarnitine is a medium-chain fatty acid (octanoic acid: eight-carbon saturated fatty acid) covalently linked to carnitine, frequently applied as a substrate for fatty acid oxidation (FAO) of mitochondrial preparations.

Abbreviation: Oct

Reference: pdf

MitoPedia topics: Substrate and metabolite 

Carnitine O-octanoyltransferase is an enzyme that catalyzes the chemical reaction:

octanoyl-CoA + L-carnitine ↔ CoA + L-octanoylcarnitine

Thus, the two substrates of this enzyme are octanoyl-CoA and L-carnitine, whereas its two products are CoA and L-octanoylcarnitine.

Application in HRR

Oct: Octanoylcarnitine (DL-Octanoyl-carnitine-HCL, C₁₅H₃₀NO₄Cl); TOCRIS Bioscience, Cat. No. 0605, 50 mg, store at R.T, desiccate); FW = 323.85.

Alternative: (±)-Octanoylcarnitine chloride, BioTrend, Cat. No. BS0136, 50 mg, store desiccate at RT; FW = 323.86.

Caution: Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.

Preparation of 100 mM stock solution (dissolved in H₂O):

1. Weigh 32.4 mg of octanoylcarnitine and dissolve in 1 ml H₂O.
2. Divide into 0.2 ml portions.
3. Store at -20 °C.

Oxygraph-2k manual titrations into 2 ml O2k-chamber

• Titration volume: 4 µl using a 10 µl syringe.
• Final concentration: 0.2 mM in MiR05 or MiR06. Optimum Oct concentration has to be evaluated, particularly in media with BSA concentrations different from MiR06.