Difference between revisions of "Octanoylcarnitine"

Revision as of 09:34, 3 September 2014

high-resolution terminology - matching measurements at high-resolution

Octanoylcarnitine

Description

Octanoylcarnitine is a medium-chain fatty acid (octanoic acid: eight-carbon saturated fatty acid) covalently linked to carnitine.

Abbreviation: Oct

Reference: pdf

MitoPedia topics: Substrate and metabolite

Carnitine O-octanoyltransferase is an enzyme that catalyzes the chemical reaction:

octanoyl-CoA + L-carnitine ↔ CoA + L-octanoylcarnitine

Thus, the two substrates of this enzyme are octanoyl-CoA and L-carnitine, whereas its two products are CoA and L-octanoylcarnitine.

Application in HRR

Oct: Octanoylcarnitine (DL-Octanoyl-carnitine-HCL, C₁₅H₃₀NO₄Cl); TOCRIS Bioscience, Cat. No. 0605, 50 mg, store at R.T, desiccate); FW = 323.85.

Alternative: (±)-Octanoylcarnitine chloride, BioTrend, Cat. No. BS0136, 50 mg, store desiccate at RT; FW = 323.86.

Caution: Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.

Preparation of 100 mM stock solution (dissolved in H₂O):

Weigh 32.4 mg of octanoylcarnitine and dissolve in 1 ml H₂O.
Divide into 0.2 ml portions.
Store at -20 °C.

Oxygraph-2k manual titrations into 2 ml O2k-chamber

Titration volume: 4 µl using a 10 µl syringe.
Final concentration: 0.2 mM in MiR05 or MiR06. Optimum Oct concentration has to be evaluated, particularly in media with BSA concentrations different from MiR06.

@@ Line 1: / Line 1: @@
 {{MitoPedia
 |abbr=Oct
-|description='''Octanoyl carnitine''' is a medium-chain fatty acid (octanoic acid: eight-carbon saturated fatty acid) covalently linked to [[carnitine]].
+|description='''Octanoylcarnitine''' is a medium-chain fatty acid (octanoic acid: eight-carbon saturated fatty acid) covalently linked to [[carnitine]].
 |info=[http://www.jbc.org/content/256/16/8371.full.pdf  ''pdf'']
 |type=Substrate ETS
+}}
+{{MitoPedia methods|type=Substrate ETS
 }}
 {{MitoPedia topics
@@ Line 9: / Line 11: @@
 |type=Substrate ETS
 }}
 '''Carnitine O-octanoyltransferase''' is an enzyme that catalyzes the chemical reaction:
 ::''octanoyl-CoA + L-carnitine ↔ CoA + L-octanoylcarnitine''
@@ Line 20: / Line 20: @@
 == Application in [[HRR]] ==
-'''Oct: Octanoyl carnitine''' (DL-Octanoyl-carnitine-HCL, C<sub>15</sub>H<sub>30</sub>NO<sub>4</sub>Cl); TOCRIS Bioscience, Cat. No. 0605, 50 mg, store at R.T, desiccate); FW = 323.85.
+'''Oct: Octanoylcarnitine''' (DL-Octanoyl-carnitine-HCL, C<sub>15</sub>H<sub>30</sub>NO<sub>4</sub>Cl); TOCRIS Bioscience, Cat. No. 0605, 50 mg, store at R.T, desiccate); FW = 323.85.
 '''Alternative:''' (±)-Octanoylcarnitine chloride, BioTrend, Cat. No. BS0136, 50 mg, store desiccate at RT; FW = 323.86.
@@ Line 30: / Line 30: @@
 '''Preparation of 100 mM stock solution''' (dissolved in H<sub>2</sub>O):
-::1) weigh 32.4 mg of octanoyl carnitine and dissolve in 1 ml H<sub>2</sub>O
+::# Weigh 32.4 mg of octanoylcarnitine and dissolve in 1 ml H<sub>2</sub>O.
-::2) divide into 0.2 ml portions
+::# Divide into 0.2 ml portions.
-::3) store frozen at -20 °C.
+::# Store at -20 °C.
-'''Oxygraph-2k Manual Titrations:'''
+'''Oxygraph-2k manual titrations into 2 ml O2k-chamber'''
-::* Titration volume: 4 µl using a 10 µl syringe
+::* Titration volume: 4 µl using a 10 µl syringe.
-::* Final conc. in 2 ml O2k-chamber: 0.2 mM
+::* Final concentration: 0.2 mM in MiR05 or [[MiR06]]. Optimum Oct concentration has to be evaluated, particularly in media with BSA concentrations different from MiR06.