Octanoylcarnitine

high-resolution terminology - matching measurements at high-resolution

Octanoylcarnitine

Description

Octanoyl carnitine is a medium-chain fatty acid (octanoic acid: eight-carbon saturated fatty acid) covalently linked to carnitine.

Abbreviation: Oct

Reference: pdf

MitoPedia topics: Substrate and metabolite 

Carnitine O-octanoyltransferase is an enzyme that catalyzes the chemical reaction:

octanoyl-CoA + L-carnitine ↔ CoA + L-octanoylcarnitine

Thus, the two substrates of this enzyme are octanoyl-CoA and L-carnitine, whereas its two products are CoA and L-octanoylcarnitine.

Application in HRR

Oct: Octanoyl carnitine (DL-Octanoyl-carnitine-HCL, C₁₅H₃₀NO₄Cl); TOCRIS Bioscience, Cat. No. 0605, 50 mg, store at R.T, desiccate); FW = 323.85.

Alternative: (±)-Octanoylcarnitine chloride, BioTrend, Cat. No. BS0136, 50 mg, store desiccate at RT; FW = 323.86.

Caution: Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.

Preparation of 100 mM stock solution (dissolved in H₂O):

1) weigh 32.4 mg of octanoyl carnitine and dissolve in 1 ml H₂O

2) divide into 0.2 ml portions

3) store frozen at -20 °C.

Oxygraph-2k Manual Titrations:

• Titration volume: 4 µl using a 10 µl syringe
• Final conc. in 2 ml O2k-chamber: 0.2 mM