Difference between revisions of "Octanoylcarnitine"
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::2) divide into 0.2 ml portions | ::2) divide into 0.2 ml portions | ||
::3) store frozen at -20 °C. | ::3) store frozen at -20 °C. | ||
'''Oxygraph-2k Manual Titrations:''' | |||
::* Titration volume: 4 µl using a 10 µl syringe | |||
::* Final conc. in 2 ml O2k-chamber: 0.2 mM | |||
Revision as of 10:06, 30 October 2013
- high-resolution terminology - matching measurements at high-resolution
Octanoylcarnitine
Description
Octanoyl carnitine is a medium-chain fatty acid (octanoic acid: eight-carbon saturated fatty acid) covalently linked to carnitine.
Abbreviation: Oct
Reference: pdf
MitoPedia topics:
Substrate and metabolite
Carnitine O-octanoyltransferase is an enzyme that catalyzes the chemical reaction:
- octanoyl-CoA + L-carnitine ↔ CoA + L-octanoylcarnitine
Thus, the two substrates of this enzyme are octanoyl-CoA and L-carnitine, whereas its two products are CoA and L-octanoylcarnitine.
Application in HRR
Oct: Octanoyl carnitine (DL-Octanoyl-carnitine-HCL, C15H30NO4Cl); TOCRIS Bioscience, Cat. No. 0605, 50 mg, store at R.T, desiccate); FW = 323.85.
Alternative: (±)-Octanoylcarnitine chloride, BioTrend, Cat. No. BS0136, 50 mg, store desiccate at RT; FW = 323.86.
Caution: Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.
Preparation of 100 mM stock solution (dissolved in H2O):
- 1) weigh 32.4 mg of octanoyl carnitine and dissolve in 1 ml H2O
- 2) divide into 0.2 ml portions
- 3) store frozen at -20 °C.
Oxygraph-2k Manual Titrations:
- Titration volume: 4 µl using a 10 µl syringe
- Final conc. in 2 ml O2k-chamber: 0.2 mM
