O2k-Catalogue: O2k-Modules: Difference between revisions
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|description='''4'''#### '''''O2k-Catalogue: O2k-Modules'''''. O2k-Modules can be obtained with the O2k or added later, and can be simply installed by the user. | |||
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|product type=O2k, MultiSensor, | |||
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[[File:O2k-Core to FluoRespirometer.jpg|right|400px |O2k-Core to FluoRespirometer]] Β | |||
== O2k-Modules == | |||
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'''O2k-Modules''' are add-on modules supported by the O2k. When one (or more) analytical parameters are monitored simultaneously with oxygen concentration and oxygen flux in the O2k, this is an '''O2k-MultiSensor''' application.Β The O2k fully supports the O2k-Modules. | |||
* [[MiPNet17.05 O2k-Fluo LED2-Module]] | |||
* [[O2k-TPP%2B_ISE-Module]] | |||
* [[O2k-pH ISE-Module]] | |||
* [[O2k-NO Amp-Module]] | |||
* [[Hydrogen peroxide]] | |||
* [[Cytochrome Spectra and Oxygen]] | |||
[[High-resolution respirometry]] is extended by O2k-MultiSensor modules allowing the simultaneous measurement of oxygen consumption and one additional parameter. Fluorometric methods are available for a wide range of analytical parameters of major interest in mitochochondrial physiology: [[O2k-Publications:_O2k-Fluorometry_-_Fluorophores|H<sub>2</sub>O<sub>2</sub> production, mitochondrial membrane potential, intracellular pH, Ca<sup>2+</sup>, Mg<sup>2+</sup>, and NADH levels]]. | |||
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Advantages of the simultaneous measurement of additional parameters in a single respirometric chamber: | |||
# Quality control of preparations by respirometric performance. | |||
# Objective exclusion criteria for protocol related problems, e.g. overtitration of uncouplers, side effects of TPP<sup>+</sup> or fluorophores. | |||
# Elimination of artifacts of normalization for a mitochondrial marker when combining two methods in a single chamber. | |||
# Additional information is acquired for a limited amount of sample. | |||
# Oxygen dependence of parameters can be studied. | |||
== ''' | == '''O2k-Catalogue: O2k-Modules''' == | ||
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== O2k-MultiSensor Manual == | |||
{{#ask:[[Category:Publications]] [[Instrument and method::O2k-Manual]] [[Additional label::O2k-MultiSensor]] | |||
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: Β»[[O2k-Publications: O2k-Fluorometry]] | |||
== References == | |||
:::* Makrecka-Kuka M, Krumschnabel G, Gnaiger E (2015) High-resolution respirometry for simultaneous measurement of oxygen and hydrogen peroxide fluxes in permeabilized cells, tissue homogenate and isolated mitochondria. Biomolecules 5:1319-38. - [[Makrecka-Kuka 2015 Biomolecules |Β»Bioblast linkΒ«]] | |||
:::* Krumschnabel G, Fontana-Ayoub M, Sumbalova Z, Heidler J, Gauper K, Fasching M, Gnaiger E (2015) Simultaneous high-resolution measurement of mitochondrial respiration and hydrogen peroxide production. Methods Mol Biol 1264:245-61. - [[Krumschnabel 2015 Methods Mol Biol |Β»Bioblast linkΒ«]] |
Latest revision as of 17:10, 15 November 2019
O2k-Catalogue: O2k-Modules
Description | 4#### O2k-Catalogue: O2k-Modules. O2k-Modules can be obtained with the O2k or added later, and can be simply installed by the user. |
---|---|
Product ID | 4#### |
Type | O2k, MultiSensor |
Link | |
Image | 4#### |
O2k-Modules
O2k-Modules are add-on modules supported by the O2k. When one (or more) analytical parameters are monitored simultaneously with oxygen concentration and oxygen flux in the O2k, this is an O2k-MultiSensor application. The O2k fully supports the O2k-Modules.
- MiPNet17.05 O2k-Fluo LED2-Module
- O2k-TPP+_ISE-Module
- O2k-pH ISE-Module
- O2k-NO Amp-Module
- Hydrogen peroxide
- Cytochrome Spectra and Oxygen
High-resolution respirometry is extended by O2k-MultiSensor modules allowing the simultaneous measurement of oxygen consumption and one additional parameter. Fluorometric methods are available for a wide range of analytical parameters of major interest in mitochochondrial physiology: H2O2 production, mitochondrial membrane potential, intracellular pH, Ca2+, Mg2+, and NADH levels.
Advantages of the simultaneous measurement of additional parameters in a single respirometric chamber:
- Quality control of preparations by respirometric performance.
- Objective exclusion criteria for protocol related problems, e.g. overtitration of uncouplers, side effects of TPP+ or fluorophores.
- Elimination of artifacts of normalization for a mitochondrial marker when combining two methods in a single chamber.
- Additional information is acquired for a limited amount of sample.
- Oxygen dependence of parameters can be studied.
O2k-Catalogue: O2k-Modules
O2k-MultiSensor Manual
Chapter | Section | Last update |
---|---|---|
MiPNet15.03 O2k-MultiSensor-ISE | O2k-MultiSensor system with ion selective electrodes (ISE). | 2021-08-02 |
MiPNet15.05 NO-manual | O2k-MultiSensor system with amperometric sensors: NO, H2S, H2O2. | 2016-04-21 |
MiPNet17.05 O2k-Fluo LED2-Module | O2k-Fluo LED2-Module. | 2021-06-22 |
MiPNet23.15 O2k-pH ISE-Module | Measurement of proton flux with the O2k-pH ISE-Module. | 2018-09-08 |
MiPNet24.12 NextGen-O2k: Q-Module | NextGen-O2k: Q-Module manual | 2021-10-29 |
MiPNet26.11 NextGen-O2k: PB-Module | NextGen-O2k: PB-Module manual | 2021-10-29 |
MiPNet26.12 NextGen-O2k: NADH-Module | NextGen-O2k: NADH-Module manual | 2022-05-17 |
MiPNet28.09 O2k-Fluo Smart-Module manual | O2k-Fluo Smart-Module manual | 2023-09-25 |
References
- Makrecka-Kuka M, Krumschnabel G, Gnaiger E (2015) High-resolution respirometry for simultaneous measurement of oxygen and hydrogen peroxide fluxes in permeabilized cells, tissue homogenate and isolated mitochondria. Biomolecules 5:1319-38. - Β»Bioblast linkΒ«
- Krumschnabel G, Fontana-Ayoub M, Sumbalova Z, Heidler J, Gauper K, Fasching M, Gnaiger E (2015) Simultaneous high-resolution measurement of mitochondrial respiration and hydrogen peroxide production. Methods Mol Biol 1264:245-61. - Β»Bioblast linkΒ«