High-resolution respirometry

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MitoPedia

High-resolution respirometry

Description

O2k-FluoRespirometer

High-resolution respirometry, HRR, is the state-of-the-art approach in mitochondria and cell research to measure respiration in mitochondrial preparations and intact cells combined with MultiSensor modules. Mitochondrial function and dysfunction have gained an increasing interest over the past years, reflecting growing awareness of the fact that mitochondria play a pivotal role in human health and disease. The most advanced way to analyze mitochondrial function is by means of high-resolution respirometry with the Oroboros O2k. Substrate-uncoupler-inhibitor titration (SUIT) protocols allow the diagnosis of numerous mitochondrial pathway and coupling defects in a single respirometric assay. Technologically, HRR is based on the Oroboros O2k, combining optimized chamber design, application of oxygen-tight materials, electrochemical sensors, Peltier-temperature control, and specially developed software features (DatLab) to obtain the unique sensitive and quantitative resolution of oxygen concentration and oxygen flux, with both, a closed-chamber or open-chamber mode of operation (TIP2k). Standardized calibration of the polarographic oxygen sensor (static sensor calibration), calibration of the sensor response time (dynamic sensor calibration), and evaluation of instrumental background oxygen flux (systemic flux compensation) provide the experimental basis for high accuracy of quantitative results and quality control in HRR. HRR can be extended for MultiSensor analysis by using the O2k-FluoRespirometer. Smart Fluo-Sensors are integrated into the O2k to measure simultaneously fluorometric signals using specific fluorophores. Potentiometric modules are available with ion selective electrodes (pH, TPP+). The NextGen-O2k is the all-in-one device including the Q-redox sensor and a PhotoBiology (PB) module.

Abbreviation: HRR

Reference: Oroboros O2k
O2k-Core to FluoRespirometer

MitoPedia methods: Respirometry, Fluorometry, Spectrophotometry 


MitoPedia O2k and high-resolution respirometry: O2k hardware 

  • Power O2k-Respirometer: Several O2k units are operated simultaneously (P1, P2, ..) to combine high-resolution respirometry with high-output.


MitoPedia: O2k and high-resolution respirometry

» MitoPedia: O2k hardware
» MitoPedia: DatLab
» MitoPedia: SUIT


Links

» MitoPedia: Respiratory states OXPHOS ROUTINE ET-capacity LEAK - ROX
» Respirometry
» MitoPedia: Respirometry
» Wikipedia: Respirometry
» O2k Applications


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O2k-Publications in the MiPMap


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