MiRK03: Difference between revisions

Revision as of 12:24, 4 May 2016

high-resolution terminology - matching measurements at high-resolution

MiRK03

Description

Mitochondrial respiration medium, MiRK03, modified after a medium described by Komary 2010 Biochim Biophys Acta, intended for use as medium for H2O2 production measurement with Amplex Red.

Abbreviation: MiRK03

Reference: Krumschnabel 2014 Methods Mol Biol, Komary 2010 Biochim Biophys Acta

MitoPedia topics: Media for respirometry

MiRK03

substance	MiRK03	molecular weight	for 1 l
EGTA	0.5 mM	380.35 g/mol	190.18 mg
MgCl₂ x 6 H₂O	3 mM	203.30 g/mol	609.90 mg
HEPES	20 mM	238.31 g/mol	4 766.2 mg
KCl	130 mM	74.56 g/mol	9 692.8 mg
KH₂PO₄	10 mM	136.09 g/mol	1 360.9 mg
BSA	1 mg/ml	-	1000 mg

MiRK03 ph - temperature dependence

data recorded with MiRK03 without BSA (only averaged values are shown; two measurements were conducted)

temperature [°C]	pH of MiRK03
20	7.15
25	7.12
30	7.09
35	7.05
37	7.03

For comparison see: Mitochondrial respiration media: comparison

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 {{MitoPedia
 |abbr=MiRK03
-|description='''Mitochondrial respiration medium, MiRL03''', modified after a medium described by [[Komary 2010 Biochim Biophys Acta]], intended for use as medium for H2O2 production measurement with Amplex Red.
+|description='''Mitochondrial respiration medium, MiRK03''', modified after a medium described by [[Komary 2010 Biochim Biophys Acta]], intended for use as medium for H2O2 production measurement with Amplex Red.
 |info=[http://bioblast.at/index.php/Krumschnabel_2014_Methods_Mol_Biol Krumschnabel 2014 Methods Mol Biol], [http://bioblast.at/index.php/Komary_2010_Biochim_Biophys_Acta Komary 2010 Biochim Biophys Acta]
 |type=Chemicals