Difference between revisions of "Krumschnabel 2016a Abstract MitoFit Science Camp 2016"
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== Affiliations == | == Affiliations == | ||
1-Oroboros Instruments, Innsbruck; 2-D. Swarovski Research Lab, Dept Visceral, Transplant and Thoracic Surgery, Innsbruck Med Univ, Austria. | 1-Oroboros Instruments, Innsbruck; 2-D. Swarovski Research Lab, Dept Visceral, Transplant and Thoracic Surgery, Innsbruck Med Univ, Austria. |
Revision as of 10:00, 8 October 2019
O2k-Protocols: Mitochondrial preparations for HRR. |
Link: MiPNet21.13
Doerrier C, Eigentler A, Krautschneider M, Schoepf B, Fontana-Ayoub M, Krumschnabel G, Gnaiger E (2016)
Event: MitoFit Science Camp 2016 Kuehtai AT
Mitochondrial (mt) preparations typically applied in high-resolution respirometry (HRR) comprise isolated mitochondria (Imt), tissue homogenates (Thom), and permeabilized tissue fibres (Pfi), each of which has its pros and cons. Here, different mt preparations from two mammalian experimental model systems, mouse and beef heart, are evaluated and compared regarding their suitability in HRR. It is shown that different mt preparations may result in almost identical (mouse) or at least comparable (beef) mitochondrial coupling control and respiratory capacities. Further, we show that homogenates produced with the PBI-Shredder provide high quality mt preparations, which may be quickly prepared with very little sample required.
β’ O2k-Network Lab: AT Innsbruck Oroboros, AT Innsbruck Gnaiger E
Labels: MiParea: Respiration, Instruments;methods, Comparative MiP;environmental MiP
Organism: Mouse
Tissue;cell: Heart
Preparation: Permeabilized tissue, Homogenate, Isolated mitochondria
Coupling state: LEAK, OXPHOS, ET
Pathway: N, S, NS, ROX
HRR: Oxygraph-2k
Event: D1
MitoFit Science Camp 2016
Affiliations
1-Oroboros Instruments, Innsbruck; 2-D. Swarovski Research Lab, Dept Visceral, Transplant and Thoracic Surgery, Innsbruck Med Univ, Austria.