Difference between revisions of "Kidane 1997 J Thermal Anal"
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{{Publication | {{Publication | ||
|title=Kidane AH, Guan Y, Evans PM, Kaderbhai MA, Kemp RB (1997) Comparison of heat flux in wild-type and genetically-engineered Chinese hamster ovary cells. J | |title=Kidane AH, Guan Y, Evans PM, Kaderbhai MA, Kemp RB (1997) Comparison of heat flux in wild-type and genetically-engineered Chinese hamster ovary cells. J Thermal Anal 49: 771-783. | ||
|info=[http://www.springerlink.com/content/tq15016745g42214/?p=e2dfabedc5644020b23812c2b57bd39d&pi=13 | |info=[http://www.springerlink.com/content/tq15016745g42214/?p=e2dfabedc5644020b23812c2b57bd39d&pi=13 doi10.1007/BF01996760] | ||
|authors=Kidane AH, Guan Y, Evans PM, Kaderbhai MA, Kemp RB | |authors=Kidane AH, Guan Y, Evans PM, Kaderbhai MA, Kemp RB | ||
|year=1997 | |year=1997 | ||
|journal=J | |journal=J Thermal Anal | ||
|abstract=It is claimed, though not without dispute, that genetically engineered mammalian cells grow more slowly than their progenitor cells because the recombinant gene system causes a metabolic burden. This was found to be the case for CHO cells transfected with expression vectors forcytochrome b5. The slower growth was associated with lower metabolic activity measured by heat flux and mitochondrial activity (rhodamine 123 fluorescence). The calorimetric-respirometric ratio was similar for all cell types, implying that the greater fluxes of glucose and glutamine in the recombinant cells was channelled to biosynthesis. This demand probably restricted the supply of pyruvate to the mitochondria in these cells. | |abstract=It is claimed, though not without dispute, that genetically engineered mammalian cells grow more slowly than their progenitor cells because the recombinant gene system causes a metabolic burden. This was found to be the case for CHO cells transfected with expression vectors forcytochrome b5. The slower growth was associated with lower metabolic activity measured by heat flux and mitochondrial activity (rhodamine 123 fluorescence). The calorimetric-respirometric ratio was similar for all cell types, implying that the greater fluxes of glucose and glutamine in the recombinant cells was channelled to biosynthesis. This demand probably restricted the supply of pyruvate to the mitochondria in these cells. | ||
|keywords=Cytochrome bΒ 5, Genetically-engineered cells, Heat flux, Metabolic burden, Mitochondrial activity, hamster | |keywords=Cytochrome bΒ 5, Genetically-engineered cells, Heat flux, Metabolic burden, Mitochondrial activity, hamster | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
|area=Respiration, Genetic knockout;overexpression | |||
|organism=Other mammals | |||
|tissues=CHO | |||
|preparations=Intact cells | |||
|couplingstates=ROUTINE | |||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
}} | }} |
Latest revision as of 10:27, 9 November 2016
Kidane AH, Guan Y, Evans PM, Kaderbhai MA, Kemp RB (1997) Comparison of heat flux in wild-type and genetically-engineered Chinese hamster ovary cells. J Thermal Anal 49: 771-783. |
Kidane AH, Guan Y, Evans PM, Kaderbhai MA, Kemp RB (1997) J Thermal Anal
Abstract: It is claimed, though not without dispute, that genetically engineered mammalian cells grow more slowly than their progenitor cells because the recombinant gene system causes a metabolic burden. This was found to be the case for CHO cells transfected with expression vectors forcytochrome b5. The slower growth was associated with lower metabolic activity measured by heat flux and mitochondrial activity (rhodamine 123 fluorescence). The calorimetric-respirometric ratio was similar for all cell types, implying that the greater fluxes of glucose and glutamine in the recombinant cells was channelled to biosynthesis. This demand probably restricted the supply of pyruvate to the mitochondria in these cells. β’ Keywords: Cytochrome b 5, Genetically-engineered cells, Heat flux, Metabolic burden, Mitochondrial activity, hamster
Labels: MiParea: Respiration, Genetic knockout;overexpression
Organism: Other mammals
Tissue;cell: CHO
Preparation: Intact cells
Coupling state: ROUTINE
HRR: Oxygraph-2k