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Kidane 1997 J Thermal Anal

From Bioblast
Publications in the MiPMap
Kidane AH, Guan Y, Evans PM, Kaderbhai MA, Kemp RB (1997) Comparison of heat flux in wild-type and genetically-engineered Chinese hamster ovary cells. J Thermal Anal 49: 771-783.

Β» doi10.1007/BF01996760

Kidane AH, Guan Y, Evans PM, Kaderbhai MA, Kemp RB (1997) J Thermal Anal

Abstract: It is claimed, though not without dispute, that genetically engineered mammalian cells grow more slowly than their progenitor cells because the recombinant gene system causes a metabolic burden. This was found to be the case for CHO cells transfected with expression vectors forcytochrome b5. The slower growth was associated with lower metabolic activity measured by heat flux and mitochondrial activity (rhodamine 123 fluorescence). The calorimetric-respirometric ratio was similar for all cell types, implying that the greater fluxes of glucose and glutamine in the recombinant cells was channelled to biosynthesis. This demand probably restricted the supply of pyruvate to the mitochondria in these cells. β€’ Keywords: Cytochrome b 5, Genetically-engineered cells, Heat flux, Metabolic burden, Mitochondrial activity, hamster


Labels: MiParea: Respiration, Genetic knockout;overexpression 


Organism: Other mammals  Tissue;cell: CHO  Preparation: Intact cells 


Coupling state: ROUTINE 

HRR: Oxygraph-2k