|
Β |
(5 intermediate revisions by 3 users not shown) |
Line 1: |
Line 1: |
| {{MitoPedia
| | #REDIRECT[[Complex IV]] |
| |abbr=CIV
| |
| |description='''CIV''': Electron flow through [[Complex IV]] (cytochrome ''c'' oxidase) is measured in intact mitochondria after inhibiton of CIII by antimycin A, and addition of [[ascorbate]] (As) and the artificial substrate [[TMPD]] (Tm). Ascorbate has to be titrated first. It reduces [[TMPD]], which further reduces cytochrome ''c'', which is the substrate of CIV. Since CIV is a proton pump of the electron transfer system, the single step of CIV-linked respiration can be measured in different coupling states: ''L'', ''P'', and ''E''. Measurement of CIV activity requires uncoupler titrations to eliminate any potential control by the phosphorylation system, and a cytochrome ''c'' test to avoid any limitation by cytochrome ''c'' release. Total oxygen uptake in the ascorbate&TMPD(&c) stimulated state (Tm) has to be corrected for chemical background oxygen consumption.
| |
| |info=[[Pathway control state]], [[Gnaiger 2002 Biochem Soc Trans]]
| |
| }}
| |
| {{MitoPedia concepts
| |
| |mitopedia concept=Respiratory state, SUIT state, Recommended
| |
| }}
| |
| {{MitoPedia topics
| |
| |mitopedia topic=EAGLE
| |
| }}
| |
| Communicated by [[Gnaiger E]] 2016-01-20; edited 2016-11-07.
| |
| == CIV(L) ==
| |
| Β | |
| Β | |
| == CIV(P) ==
| |
| Β | |
| Β | |
| == CIV(E) ==
| |
| Β | |
| Β | |
| Β | |
| [[Image:MiPMap Publication.jpg|left|120px|link=http://www.bioblast.at/index.php/MiPMap|Publications in the MiPMap]]
| |
| == Publications: Complex IV single step ==
| |
| '''''Sort in ascending/descending order by a click on one of the small symbols in squares below'''''.
| |
| Default sorting: chronological. Empty fields appear first in ascending order.
| |
| {{#ask:[[Category:Publications]] [[Pathways::CIV]]
| |
| |?Was published in year=Year
| |
| |?Has title=Reference
| |
| |?Coupling states=Coupling
| |
| |?Pathways=Pathway
| |
| |?Preparation=Preparation
| |
| |?Mammal and model=Organism
| |
| |?Tissue and cell=Tissue;cell
| |
| |format=broadtable
| |
| |limit=5000
| |
| |offset=0
| |
| |sort=Was published in year
| |
| |order=descending
| |
| }}
| |
| Β | |
| [[Image:MiPMap Publication.jpg|left|120px|link=http://www.bioblast.at/index.php/MiPMap|Publications in the MiPMap]] | |
| == Abstracts: Complex IV single step ==
| |
| '''''Sort in ascending/descending order by a click on one of the small symbols in squares below'''''.
| |
| {{#ask:[[Category:Abstracts]] [[Pathways::CIV]]
| |
| |?Was submitted in year=Year
| |
| |?Has title=Reference
| |
| |?Coupling states=Coupling
| |
| |?Pathways=Pathway
| |
| |?Preparation=Preparation
| |
| |?Mammal and model=Organism
| |
| |?Tissue and cell=Tissue;cell
| |
| |format=broadtable
| |
| |limit=5000
| |
| |offset=0
| |
| |sort=Was submitted in year
| |
| |order=descending
| |
| }}
| |