Description
The oxygen signal of the O2k is transmitted from the electrochemical polarographic oxygen sensor (OroboPOS) for each of the two chambers to DatLab. The primary signal is a current [mAmp] which is converted into a voltage [V], and calibrated in SI units for amount of substrance concentration [Β΅mol.dm-3 or Β΅M].
Reference: MiPNet19.18D O2k-calibration, MiPNet06.03 POS-calibration-SOP
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MitoPedia O2k and high-resolution respirometry: O2k hardware, DatLab
MitoPedia O2k and high-resolution respirometry:
O2k-Open Support
Stability of the oxygen signal
The stability of the oxygen signal of the O2k is evaluated by the (negative) uncorrected slope over time. After smoothing, the slope of a stable sensor should be less than +- 1 pmol.s-1.ml-1 in a 2-ml chamber.
- Instability of the oxygen signal
- There is drift of the oxygen signal over short or long periods of time.
- The signal at air saturation is abnormally high for a given gain setting.
- The zero current is high (>2.5%) and may show drift.
- Follow the standard instructions for oxygen sensor service.
Oxygen signal does not respond
- The oxygen signal remains at or close to zero even at high oxygen levels.
- The signal at air saturation is abnormally low.
- Check the gain setting for the affected oxygen channel. Set to Gain 1.
- Apply technical service: O2k-technical support and open innovation