Step
|
State
|
Pathway
|
Q-junction
|
Comment - Events (E) and Marks (M)
|
ce1
|
ROUTINE
|
|
|
ce1
- ROUTINE respiration in the physiological coupling state R. Externally added permeable substrates could contribute to this respiratory state.
|
(ce2Omy)
|
L(Omy)
|
|
|
ce1;(ce2Omy)
- Non-phosphorylating resting state (LEAK state); LEAK-respiration, L(Omy), after blocking the ATP synthase with oligomycin.
- The addition of oligomycin, any other inhibitor of the ATP synthase or the adenine nucleotide translocase or its carriers (EtOH) is optional depending on the microalgal species studied. If we add only the carrier we do not reach LEAK state and we will evaluate if the EtOH alters the ROUTINE state.
|
ce3U
|
E
|
|
|
ce1;(ce2Omy);ce3U
|
ce4Rot
|
B(Rot) or ROX
|
|
|
ce1;(ce2Omy);ce3U;ce4Rot
- In microalgal cells, Rotenone inhibits Complex I (CI) but, if the internal type II NADH dehydrogenase remains active NADH-Q branch respiration, B(Rot), can continue
- Rox is the residual oxygen consumption in the ROX state, due to oxidative side reactions, estimated either after inhibition of CIII (e.g. antimycin A, myxothiazol), CIV (e.g. Cyanide) or in the absence of endogenous fuel-substrates. Rox is subtracted from oxygen flux as a baseline for all respiratory states, to obtain mitochondrial respiration.
|
ce5Ama
|
ROX
|
|
|
ce1;(ce2Omy);ce3U;ce4Rot;ce5Ama
- Rox is the residual oxygen consumption in the ROX state, due to oxidative side reactions, estimated after addition of antimycin A (inhibitor of CIII). Rox is subtracted from oxygen flux as a baseline for all respiratory states, to obtain mitochondrial respiration (mt).
|