MiPNet17.04 CitrateSynthase
Laboratory protocol: Citrate synthase a mitochondrial marker enzyme. |
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Oroboros (2020-04-18) Mitochondr Physiol Network
Abstract: Eigentler A, Draxl A, Gnaiger E (2020) Laboratory protocol: Citrate synthase a mitochondrial marker enzyme. Mitochondr Physiol Network 17.04(04):1-12.
Version 04: New Section 6. Normalization of respiratory flux for CS activity • Keywords: Normalization of rate
• O2k-Network Lab: AT Innsbruck Oroboros
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Author contributions
- This MiPNet Protocol was originally prepared by Kuznetsov AV, Lassing B, Gnaiger E as MiPNet08.14 (2003) and further edited by Wiethüchter A (2011). MiPNet17.04 (2012 version) was edited by Eigentler A, Draxl A, Gnaiger E; contribution to K-Regio project MitoCom Tyrol, funded by the Tyrolian Government and the European Regional Development Fund (ERDF).
- Last updates: contribution to H2020 Project NextGen-O2k.
- Bioblast links: Normalization - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Quantities for normalization
- » Count in contrast to Number
- » Mitochondrial marker
- » O2k-Protocols: mitochondrial and marker-enzymes
- » Citrate synthase activity
- Quantities for normalization
- General
- Related keyword lists
Labels: MiParea: Respiration, Instruments;methods
Preparation: Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, Enzyme
Enzyme: Marker enzyme
HRR: Oxygraph-2k, O2k-Protocol
Mt- and marker-enzymes, Normalization of rate