Difference between revisions of "Ydfors 2015 J Physiol"
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{{Publication | {{Publication | ||
|title=Ydfors M, Hughes MC, Laham R, Schlattner U, Norrbom J, Perry CG (2015) Modelling ''in vivo'' creatine/phosphocreatine ''in vitro'' reveal divergent adaptations in human muscle mitochondrial respiratory control by ADP after acute and chronic exercise. J Physiol | |title=Ydfors M, Hughes MC, Laham R, Schlattner U, Norrbom J, Perry CG (2015) Modelling ''in vivo'' creatine/phosphocreatine ''in vitro'' reveal divergent adaptations in human muscle mitochondrial respiratory control by ADP after acute and chronic exercise. J Physiol 594:3127-40. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/26631938 PMID: 26631938] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/26631938 PMID: 26631938] | ||
|authors=Ydfors M, Hughes MC, Laham R, Schlattner U, Norrbom J, Perry CG | |authors=Ydfors M, Hughes MC, Laham R, Schlattner U, Norrbom J, Perry CG | ||
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|journal=J Physiol | |journal=J Physiol | ||
|abstract=Mitochondrial respiratory control by ADP (K<sub>mapp</sub>) is viewed as a critical regulator of muscle energy homeostasis. However, acute exercise increases, decreases or has no effect on K<sub>mapp</sub> in human muscle whereas chronic exercise surprisingly decreases sensitivity despite greater mitochondrial content. We hypothesized modelling ''in vivo'' mitochondrial creatine kinase (mtCK)-dependent phosphate shuttling conditions ''in vitro'' would reveal increased sensitivity (lower K<sub>mapp</sub>) following acute and chronic exercise. K<sub>mapp</sub> was determined ''in vitro'' with 20 mM Cr (+Cr), 0 mM Cr (-Cr) or '''in vivo'' exercising' 20 mM Cr/2.4 mM PCr (Cr:PCr) on ''vastus lateralis'' biopsies sampled from 11 males before, immediately Post and 3hr Post exercise on the 1<sup>st</sup>, 5<sup>th</sup> and 9<sup>th</sup> sessions over 3 weeks. Dynamic responses to acute exercise occurred throughout training, whereby the 1<sup>st</sup> session did not change K<sub>mapp</sub> with ''in vivo'' Cr:PCr despite increases in -Cr. The 5<sup>th</sup> session decreased sensitivity with Cr:PCr or +Cr despite no change in -Cr. Chronic exercise increased sensitivity +/- Cr in association with increased electron transport chain content (+33-62% Complexes I-V), supporting classic proposals linking increased sensitivity to oxidative capacity. However, ''in vivo'' Cr:PCr reveals a perplexing decreased sensitivity contrasting the increases seen +/- Cr. Functional responses occurred without changes in fibre type or proteins regulating mitochondrial-cytosolic energy exchange (mtCK, VDAC, ANT). Despite the dynamic responses seen with +/- Cr, modelling ''in vivo'' phosphate shuttling conditions ''in vitro'' reveals ADP sensitivity is unchanged following high intensity exercise and is decreased after training. These findings challenge our understanding of how exercise regulates skeletal muscle energy homeostasis. | |abstract=Mitochondrial respiratory control by ADP (K<sub>mapp</sub>) is viewed as a critical regulator of muscle energy homeostasis. However, acute exercise increases, decreases or has no effect on K<sub>mapp</sub> in human muscle whereas chronic exercise surprisingly decreases sensitivity despite greater mitochondrial content. We hypothesized modelling ''in vivo'' mitochondrial creatine kinase (mtCK)-dependent phosphate shuttling conditions ''in vitro'' would reveal increased sensitivity (lower K<sub>mapp</sub>) following acute and chronic exercise. K<sub>mapp</sub> was determined ''in vitro'' with 20 mM Cr (+Cr), 0 mM Cr (-Cr) or '''in vivo'' exercising' 20 mM Cr/2.4 mM PCr (Cr:PCr) on ''vastus lateralis'' biopsies sampled from 11 males before, immediately Post and 3hr Post exercise on the 1<sup>st</sup>, 5<sup>th</sup> and 9<sup>th</sup> sessions over 3 weeks. Dynamic responses to acute exercise occurred throughout training, whereby the 1<sup>st</sup> session did not change K<sub>mapp</sub> with ''in vivo'' Cr:PCr despite increases in -Cr. The 5<sup>th</sup> session decreased sensitivity with Cr:PCr or +Cr despite no change in -Cr. Chronic exercise increased sensitivity +/- Cr in association with increased electron transport chain content (+33-62% Complexes I-V), supporting classic proposals linking increased sensitivity to oxidative capacity. However, ''in vivo'' Cr:PCr reveals a perplexing decreased sensitivity contrasting the increases seen +/- Cr. Functional responses occurred without changes in fibre type or proteins regulating mitochondrial-cytosolic energy exchange (mtCK, VDAC, ANT). Despite the dynamic responses seen with +/- Cr, modelling ''in vivo'' phosphate shuttling conditions ''in vitro'' reveals ADP sensitivity is unchanged following high intensity exercise and is decreased after training. These findings challenge our understanding of how exercise regulates skeletal muscle energy homeostasis. | ||
|mipnetlab=CA Toronto Perry CG | |keywords=Blebbistatin | ||
|mipnetlab=FR Grenoble Saks VA, FR Grenoble Schlattner U, CA Guelph Holloway GP, CA Toronto Perry CG, US NC Greenville Neufer PD | |||
}} | }} | ||
{{Labeling | {{Labeling | ||
| Line 12: | Line 13: | ||
|organism=Human | |organism=Human | ||
|tissues=Skeletal muscle | |tissues=Skeletal muscle | ||
|preparations=Permeabilized tissue | |||
|enzymes=Complex I, Complex II;succinate dehydrogenase, Complex III, Complex IV;cytochrome c oxidase, Complex V;ATP synthase | |||
|topics=ADP, PCr;Cr | |||
|couplingstates=OXPHOS | |||
|pathways=N | |||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|additional= | |additional=2016-01 | ||
}} | }} | ||
Latest revision as of 15:39, 7 November 2016
| Ydfors M, Hughes MC, Laham R, Schlattner U, Norrbom J, Perry CG (2015) Modelling in vivo creatine/phosphocreatine in vitro reveal divergent adaptations in human muscle mitochondrial respiratory control by ADP after acute and chronic exercise. J Physiol 594:3127-40. |
Ydfors M, Hughes MC, Laham R, Schlattner U, Norrbom J, Perry CG (2015) J Physiol
Abstract: Mitochondrial respiratory control by ADP (Kmapp) is viewed as a critical regulator of muscle energy homeostasis. However, acute exercise increases, decreases or has no effect on Kmapp in human muscle whereas chronic exercise surprisingly decreases sensitivity despite greater mitochondrial content. We hypothesized modelling in vivo mitochondrial creatine kinase (mtCK)-dependent phosphate shuttling conditions in vitro would reveal increased sensitivity (lower Kmapp) following acute and chronic exercise. Kmapp was determined in vitro with 20 mM Cr (+Cr), 0 mM Cr (-Cr) or 'in vivo exercising' 20 mM Cr/2.4 mM PCr (Cr:PCr) on vastus lateralis biopsies sampled from 11 males before, immediately Post and 3hr Post exercise on the 1st, 5th and 9th sessions over 3 weeks. Dynamic responses to acute exercise occurred throughout training, whereby the 1st session did not change Kmapp with in vivo Cr:PCr despite increases in -Cr. The 5th session decreased sensitivity with Cr:PCr or +Cr despite no change in -Cr. Chronic exercise increased sensitivity +/- Cr in association with increased electron transport chain content (+33-62% Complexes I-V), supporting classic proposals linking increased sensitivity to oxidative capacity. However, in vivo Cr:PCr reveals a perplexing decreased sensitivity contrasting the increases seen +/- Cr. Functional responses occurred without changes in fibre type or proteins regulating mitochondrial-cytosolic energy exchange (mtCK, VDAC, ANT). Despite the dynamic responses seen with +/- Cr, modelling in vivo phosphate shuttling conditions in vitro reveals ADP sensitivity is unchanged following high intensity exercise and is decreased after training. These findings challenge our understanding of how exercise regulates skeletal muscle energy homeostasis. • Keywords: Blebbistatin
• O2k-Network Lab: FR Grenoble Saks VA, FR Grenoble Schlattner U, CA Guelph Holloway GP, CA Toronto Perry CG, US NC Greenville Neufer PD
Labels: MiParea: Respiration, Exercise physiology;nutrition;life style
Organism: Human
Tissue;cell: Skeletal muscle
Preparation: Permeabilized tissue
Enzyme: Complex I, Complex II;succinate dehydrogenase, Complex III, Complex IV;cytochrome c oxidase, Complex V;ATP synthase
Regulation: ADP, PCr;Cr
Coupling state: OXPHOS
Pathway: N
HRR: Oxygraph-2k
2016-01
