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Difference between revisions of "User talk:Zhang Feiyuan/My sandbox 3"

From Bioblast
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I.
The Clark Oxygen Sensor


[http://www.selectscience.net/productDetails.aspx?prodID=13829&catID=490&u=0851F520-FA82-4B30-AFA0-E0265605AB71 selectscience.net]
[http://chem.ch.huji.ac.il/ Dr. Leland Clark] - inventor of the "Clark Oxygen Sensor" (1954); the Clark type polarographic oxygen sensor remains the gold standard for measuring dissolved oxygen in biomedical, environmental and industrial applications  - Obituary
<font color="#008B8B">(link,doesnt work) </font>;
[http://www.ncbi.nlm.nih.gov/pubmed/3519875?dopt=Abstract Review]




II.
Convenience and simplicity
International Society of Biological Calorimetry


ISBC: www.biocalorimetry.org


'The convenience and simplicity of the polarographic 'oxygen electrode' technicque for measuring rapid changes in the rate of oxygen utilization by cellular and subcellular systems is now leading to its more general application in many laboratories. The types and design of oxygen electrodes vary, depending on the invetigator's ingenuity and specific requirements of the system under investigation.'


BioThermoKinetics
Estabrook R (1967) Mitochondrial respiratory control and the polarographic measurement of ADP:O ratios. Methods Enzymol. 10: 41-47.


BTK: http://www.rmsb.u-bordeaux2.fr/BTK/


•See also: BTK 1994  "What is controlling Life?"
More precise information
 
"However, one approach that is underutilized in whole-cell bioenergetics, and that is accessible as long as cells can be obtained in suspension, is the oxygen electrode, which can obtain more precise information on the bioenergetic status of the in situ mitochondria than more ‘high-tech’ approaches such as fluorescent monitoring of Δψm.” 
 
Nicholls DG, Ferguson S (2002) Bioenergetics 3. Academic Press, London.

Revision as of 13:56, 20 April 2016

The Clark Oxygen Sensor


Dr. Leland Clark - inventor of the "Clark Oxygen Sensor" (1954); the Clark type polarographic oxygen sensor remains the gold standard for measuring dissolved oxygen in biomedical, environmental and industrial applications - Obituary (link,doesnt work) ; Review


Convenience and simplicity


'The convenience and simplicity of the polarographic 'oxygen electrode' technicque for measuring rapid changes in the rate of oxygen utilization by cellular and subcellular systems is now leading to its more general application in many laboratories. The types and design of oxygen electrodes vary, depending on the invetigator's ingenuity and specific requirements of the system under investigation.'

Estabrook R (1967) Mitochondrial respiratory control and the polarographic measurement of ADP:O ratios. Methods Enzymol. 10: 41-47.


More precise information

"However, one approach that is underutilized in whole-cell bioenergetics, and that is accessible as long as cells can be obtained in suspension, is the oxygen electrode, which can obtain more precise information on the bioenergetic status of the in situ mitochondria than more ‘high-tech’ approaches such as fluorescent monitoring of Δψm.”

Nicholls DG, Ferguson S (2002) Bioenergetics 3. Academic Press, London.