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| {{Product
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| |description='''Fluorescence-Control Unit (Series H)''' with Current-Control (O2k-Chamber A and B) for regulation of light intensity of the LED in the fluorescence sensors. This item is a standard component of the [[O2k-Main Unit]].
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| |product id=44100-11
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| |product type=
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| |info='''''>> More details? Consult [[O2k-Fluorescence LED2-Module]]'''''
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| |product image=[[Image:Fluorescence-Control Unit lettered.jpg|180px|right]]
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| }}
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| __TOC__
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| == O2k-Guide ==
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| '''O2k-Guide: Setup - next step - [[Fluorescence-Sensor#Select_the_Fluorescence-Sensors|Selecting a Fluorescence Sensor]]'''
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| '''O2k-Guide: Setup - next step - [[Filter-Cap#Mounting_a_Filter-Cap|Mounting a Filter-Cap]]'''
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| === The Oxygraph Window ===
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| [[file:DL5 control Amp.png|300px|right|Alt=O2k control window, Amp. section|O2k control window, Amp. section]]
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| ::::* From the DatLab menu choose [Oxygraph]/[O2k-Control].
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| :::: In the Configuration Table of the Oxygraph Control window, the used sensor can be entered for documentation purposes.
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| :::: In the Control Table of the Oxygraph Control window the LED intensity and the gain (amplification) can be set.
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| === Control of LED-intensity ===
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| :::: The light intensity of the LEDs is set by the current control, independent for each fluorescence sensor (O2k-Chamber A and B). The current is controlled by DatLab in a very wide range for optimization according to sample and fluorophore requirements. At higher LED-intensity the optical sensitivity is increased, i.e. the signal change per concentration change is enhanced. However, even moderately intensive light may exert negative effects: (i) Damage to the sample reducing the biological activity. (ii) Damage to fluorophores catalyzing degradation and various side reactions. Therefore, the LED-intensity should be kept as low as compatible with a smooth signal, i.e. when the resolution is just not limited by noise or disturbances. The values indicated in the table [[O2k-Fluo_LED2-Module#Application-specific_settings|application specific settings]] are only suggestions to start with. It is recommended to optimize the light intensity specifically for each application.
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| :::: '''Setting the LED Intensity from DatLab: O2k-Fluorescence LED2-Module Series B and higher (modules without mechanical selector switch)'''
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| ::::* Set the desired light intensity (0 to 2000) in the field "Amp Polarisation Voltage [mV]".
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| ::::* Click "Send to Oxygraph" or "Connect to Oxygraph-2k" to apply the new settings
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| ::::* If any current >= 0 is set and the Fluorescence Module is switched on, the indicator light below the respective chamber on the Fluorescence-Control Unit will be green. If the current is zero (the LED is not used) but the Fluorescence-Control_Unit is switched on the indicator light will be red.
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| :::: Limitations: For Series D oxygraphs the LED intensity has to be set to the same value for both chambers.
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| Note: The actual current (in mA) used to drive the LED is the value set in DatLab (Amp polarization voltage) divided by 100.
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| Previously we quoted the suggested current. To simplify the operation we now state directly the required DatLab settings.
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| :::: '''Setting the LED Intensity from DatLab: O2k-Fluorescence LED2-Module Series A (modules with mechanical selector switch)'''
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| :::: Series A of the O2k-Fluorescence LED2-Module has a mechanical selector switch for each chamber on the front side of the Fluorescence-Control_Unit
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| ::::* Bring the switch on the front panel of the Fluorescence- Control Unit into position 9 (use the small supplied screw driver)
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| ::::* Follow the procedure described above for Series B and higher.
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| :::: For LED2-Module Series A it is still possible to set the LED current directly at the Fluorescence-Control Unit. However, this less convenient method is obsolete. The relevant instructions can be found on the discussion page in the [[Talk:O2k-Fluo_LED2-Module#Archive:_Instructions_for_Older_Models| archive]].
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| === Gain ===
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| :::: The gain for the Amp channel can be set in the Control Table of the Oxygraph Control window, the section “Amp” to 1, 10, 100, or 1000. The gain setting will influence the Amp raw signal recorded in Volt. See [[O2k-Fluorescence_LED2-Module#Observing_the_fluorescence_signal| Observing the Fluorescence Signal]]. The amplified signal can be recorded in the range -10 to +10 V.
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| :::: The gain setting should be chosen to obtain a maximum signal well below 10 V. If the maximum observed raw signal was 9 V in an initial experiment, then the gain should be reduced to avoid "off scale" (>9.99 V). On the other hand, if the maximum recorded raw signal was considerable lower than 1 V (e.g. 0.2 V), the gain can be increased to avoid limitation of resolution by digital noise.
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| :::: Note for advanced users: At gain 1, a current of 1 nA is recorded as a voltage of 1 mV (0.001 V). At gain 100, 1 nA corresponds to 100 mV (0.1 V), as can be seen form the table below:
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| {| style="border-spacing:0;"
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| | style="border-top:0.018cm solid #000000;border-bottom:0.018cm solid #000000;border-left:0.018cm solid #000000;border-right:none;padding-top:0cm;padding-bottom:0cm;padding-left:0.191cm;padding-right:0.191cm;"| <center>Amplification</center>
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| | style="border-top:0.018cm solid #000000;border-bottom:0.018cm solid #000000;border-left:0.018cm solid #000000;border-right:none;padding-top:0cm;padding-bottom:0cm;padding-left:0.191cm;padding-right:0.191cm;"| <center>1 pA at sensor</center>
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| | style="border-top:0.018cm solid #000000;border-bottom:0.018cm solid #000000;border-left:0.018cm solid #000000;border-right:none;padding-top:0cm;padding-bottom:0cm;padding-left:0.191cm;padding-right:0.191cm;"| <center>1 mV recorded</center>
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| | style="border-top:0.018cm solid #000000;border-bottom:0.018cm solid #000000;border-left:0.018cm solid #000000;border-right:none;padding-top:0cm;padding-bottom:0cm;padding-left:0.191cm;padding-right:0.191cm;"| <center>Range</center>
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| | style="border:0.018cm solid #000000;padding-top:0cm;padding-bottom:0cm;padding-left:0.191cm;padding-right:0.191cm;"| <center>Digital resolution</center>
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| |-
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| | style="border-top:0.018cm solid #000000;border-bottom:0.018cm solid #000000;border-left:0.018cm solid #000000;border-right:none;padding-top:0cm;padding-bottom:0cm;padding-left:0.191cm;padding-right:0.191cm;"|
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| | style="border-top:0.018cm solid #000000;border-bottom:0.018cm solid #000000;border-left:0.018cm solid #000000;border-right:none;padding-top:0cm;padding-bottom:0cm;padding-left:0.191cm;padding-right:0.191cm;"| <center>mV recorded</center>
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| | style="border-top:0.018cm solid #000000;border-bottom:0.018cm solid #000000;border-left:0.018cm solid #000000;border-right:none;padding-top:0cm;padding-bottom:0cm;padding-left:0.191cm;padding-right:0.191cm;"| <center>pA at sensor</center>
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| | style="border-top:0.018cm solid #000000;border-bottom:0.018cm solid #000000;border-left:0.018cm solid #000000;border-right:none;padding-top:0cm;padding-bottom:0cm;padding-left:0.191cm;padding-right:0.191cm;"| <center>nA</center>
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| | style="border:0.018cm solid #000000;padding-top:0cm;padding-bottom:0cm;padding-left:0.191cm;padding-right:0.191cm;"| <center>pA</center>
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| | style="border-top:0.018cm solid #000000;border-bottom:0.018cm solid #000000;border-left:0.018cm solid #000000;border-right:none;padding-top:0cm;padding-bottom:0cm;padding-left:0.191cm;padding-right:0.191cm;"| <div align="right">1</div>
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| | style="border-top:0.018cm solid #000000;border-bottom:0.018cm solid #000000;border-left:0.018cm solid #000000;border-right:none;padding-top:0cm;padding-bottom:0cm;padding-left:0.191cm;padding-right:0.191cm;"| 0.001
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| | style="border-top:0.018cm solid #000000;border-bottom:0.018cm solid #000000;border-left:0.018cm solid #000000;border-right:none;padding-top:0cm;padding-bottom:0cm;padding-left:0.191cm;padding-right:0.191cm;"| <div align="right">1000 </div>
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| | style="border-top:0.018cm solid #000000;border-bottom:0.018cm solid #000000;border-left:0.018cm solid #000000;border-right:none;padding-top:0cm;padding-bottom:0cm;padding-left:0.191cm;padding-right:0.191cm;"| <div align="right">+-10000</div>
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| | style="border:0.018cm solid #000000;padding-top:0cm;padding-bottom:0cm;padding-left:0.191cm;padding-right:0.191cm;"| <div align="right">333</div>
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| | style="border-top:0.018cm solid #000000;border-bottom:0.018cm solid #000000;border-left:0.018cm solid #000000;border-right:none;padding-top:0cm;padding-bottom:0cm;padding-left:0.191cm;padding-right:0.191cm;"| <div align="right">10</div>
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| | style="border-top:0.018cm solid #000000;border-bottom:0.018cm solid #000000;border-left:0.018cm solid #000000;border-right:none;padding-top:0cm;padding-bottom:0cm;padding-left:0.191cm;padding-right:0.191cm;"| 0.01
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| | style="border-top:0.018cm solid #000000;border-bottom:0.018cm solid #000000;border-left:0.018cm solid #000000;border-right:none;padding-top:0cm;padding-bottom:0cm;padding-left:0.191cm;padding-right:0.191cm;"| <div align="right">100 </div>
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| | style="border-top:0.018cm solid #000000;border-bottom:0.018cm solid #000000;border-left:0.018cm solid #000000;border-right:none;padding-top:0cm;padding-bottom:0cm;padding-left:0.191cm;padding-right:0.191cm;"| <div align="right">+-1000</div>
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| | style="border:0.018cm solid #000000;padding-top:0cm;padding-bottom:0cm;padding-left:0.191cm;padding-right:0.191cm;"| <div align="right">33</div>
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| |-
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| | style="border-top:0.018cm solid #000000;border-bottom:0.018cm solid #000000;border-left:0.018cm solid #000000;border-right:none;padding-top:0cm;padding-bottom:0cm;padding-left:0.191cm;padding-right:0.191cm;"| <div align="right">1000</div>
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| | style="border-top:0.018cm solid #000000;border-bottom:0.018cm solid #000000;border-left:0.018cm solid #000000;border-right:none;padding-top:0cm;padding-bottom:0cm;padding-left:0.191cm;padding-right:0.191cm;"| 1
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| | style="border-top:0.018cm solid #000000;border-bottom:0.018cm solid #000000;border-left:0.018cm solid #000000;border-right:none;padding-top:0cm;padding-bottom:0cm;padding-left:0.191cm;padding-right:0.191cm;"| <div align="right">1 </div>
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| | style="border-top:0.018cm solid #000000;border-bottom:0.018cm solid #000000;border-left:0.018cm solid #000000;border-right:none;padding-top:0cm;padding-bottom:0cm;padding-left:0.191cm;padding-right:0.191cm;"| <div align="right">+-10</div>
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| | style="border:0.018cm solid #000000;padding-top:0cm;padding-bottom:0cm;padding-left:0.191cm;padding-right:0.191cm;"| <div align="right">0.3</div>
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| '''O2k-Guide: [[O2k-Fluorescence_LED2-Module#DatLab-Analysis]]'''
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| [[Category:Technical service]]
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