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Difference between revisions of "Trimmer 2009 Mol Neurodegener"

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{{Publication
{{Publication
|title=Trimmer PA, Schwartz KM, Borland MK, De Taboada L, Streeter J, Oron U (2009) Reduced axonal transport in Parkinson's disease cybrid neurites is restored by light therapy. Mol. Neurodegener. 4: 26.
|title=Trimmer PA, Schwartz KM, Borland MK, De Taboada L, Streeter J, Oron U (2009) Reduced axonal transport in Parkinson's disease cybrid neurites is restored by light therapy. Mol. Neurodegener. 4: 26.
|info=[http://www.ncbi.nlm.nih.gov/pubmed/19534794 PMID: 19534794]
|authors=Trimmer PA, Schwartz KM, Borland MK, De Taboada L, Streeter J, Oron U
|authors=Trimmer PA, Schwartz KM, Borland MK, De Taboada L, Streeter J, Oron U
|year=2009
|year=2009
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to improve neuronal function in patients with PD.
to improve neuronal function in patients with PD.
|keywords=Parkinson's disease,Β  Mitochondria, Cybrid, Respiration,Β  Axonal transport, Complex I,Β  Gene expression
|keywords=Parkinson's disease,Β  Mitochondria, Cybrid, Respiration,Β  Axonal transport, Complex I,Β  Gene expression
|info=[http://www.ncbi.nlm.nih.gov/pubmed/19534794 PMID: 19534794]
|discipline=Biomedicine
}}
}}
{{Labeling
{{Labeling
|discipline=Biomedicine
|instruments=Oxygraph-2k
|injuries=Mitochondrial Disease; Degenerative Disease and Defect, Genetic Defect; Knockdown; Overexpression
|injuries=Mitochondrial Disease; Degenerative Disease and Defect
|organism=Human
|organism=Human
|tissues=Neurons; Brain
|tissues=Neurons; Brain
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|enzymes=Complex I
|enzymes=Complex I
|topics=Respiration; OXPHOS; ETS Capacity, Mitochondrial Biogenesis; Mitochondrial Density
|topics=Respiration; OXPHOS; ETS Capacity, Mitochondrial Biogenesis; Mitochondrial Density
|instruments=Oxygraph-2k
|discipline=Biomedicine
}}
}}

Revision as of 14:04, 17 November 2011

Publications in the MiPMap
Trimmer PA, Schwartz KM, Borland MK, De Taboada L, Streeter J, Oron U (2009) Reduced axonal transport in Parkinson's disease cybrid neurites is restored by light therapy. Mol. Neurodegener. 4: 26.

Β» PMID: 19534794

Trimmer PA, Schwartz KM, Borland MK, De Taboada L, Streeter J, Oron U (2009) Mol. Neurodegener.

Abstract: Background: It has been hypothesized that reduced axonal transport contributes to the degeneration of neuronal processes in Parkinson's disease (PD). Mitochondria supply the adenosine triphosphate (ATP) needed to support axonal transport and contribute to many other cellular functions essential for the survival of neuronal cells. Furthermore, mitochondria in PD tissues are metabolically and functionally compromised. To address this hypothesis, we measured the velocity of mitochondrial movement in human transmitochondrial cybrid "cytoplasmic hybrid" neuronal cells bearing mitochondrial DNA from patients with sporadic PD and disease-free age-matched volunteer controls (CNT). The absorption of low level, near-infrared laser light by components of the mitochondrial electron transport chain (mtETC) enhances mitochondrial metabolism, stimulates oxidative phosphorylation and improves redox capacity. PD and CNT cybrid neuronal cells were exposed to near-infrared laser light to determine if the velocity of mitochondrial movement can be restored by low level light therapy (LLLT). Axonal transport of labeled mitochondria was documented by time lapse microscopy in dopaminergic PD and CNT cybrid neuronal cells before and after illumination with an 810 nm diode laser (50 mW/cm2 ) for 40 seconds. Oxygen utilization and assembly of mtETC complexes were also determined.

Results: The velocity of mitochondrial movement in PD cybrid neuronal cells (0.175 +/- 0.005 SEM) was significantly reduced (p < 0.02) compared to mitochondrial movement in disease free CNT cybrid neuronal cells (0.232 +/- 0.017 SEM). For two hours after LLLT, the average velocity of mitochondrial movement in PD cybrid neurites was significantly (p < 0.003) increased (to 0.224 +/- 0.02 SEM) and restored to levels comparable to CNT. Mitochondrial movement in CNT cybrid neurites was unaltered by LLLT (0.232 +/- 0.017 SEM). Assembly of complexes in the mtETC was reduced and oxygen utilization was altered in PD cybrid neuronal cells. PD cybrid neuronal cell lines with the most dysfunctional mtETC assembly and oxygen utilization profiles were least responsive to LLLT.

Conclusion: The results from this study support our proposal that axonal transport is reduced in sporadic PD and that a single, brief treatment with near-infrared light can restore axonal transport to control levels. These results are the first demonstration that LLLT can increase axonal transport in model human dopaminergic neuronal cells and they suggest that LLLT could be developed as a novel treatment to improve neuronal function in patients with PD. β€’ Keywords: Parkinson's disease, Mitochondria, Cybrid, Respiration, Axonal transport, Complex I, Gene expression


Labels:

Stress:Mitochondrial Disease; Degenerative Disease and Defect"Mitochondrial Disease; Degenerative Disease and Defect" is not in the list (Cell death, Cryopreservation, Ischemia-reperfusion, Permeability transition, Oxidative stress;RONS, Temperature, Hypoxia, Mitochondrial disease) of allowed values for the "Stress" property.  Organism: Human  Tissue;cell: Neurons; Brain"Neurons; Brain" is not in the list (Heart, Skeletal muscle, Nervous system, Liver, Kidney, Lung;gill, Islet cell;pancreas;thymus, Endothelial;epithelial;mesothelial cell, Blood cells, Fat, ...) of allowed values for the "Tissue and cell" property.  Preparation: Intact Cell; Cultured; Primary"Intact Cell; Cultured; Primary" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.  Enzyme: Complex I  Regulation: Respiration; OXPHOS; ETS Capacity"Respiration; OXPHOS; ETS Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property., Mitochondrial Biogenesis; Mitochondrial Density"Mitochondrial Biogenesis; Mitochondrial Density" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property. 


HRR: Oxygraph-2k