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Difference between revisions of "Template talk:Next oroboros ecosystem agenda"

From Bioblast
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::::[[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] Do you know how to evaluate '''fatty acid oxidation''' avoiding to overestimate it?'''
::::[[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] Do you know how to evaluate '''[[Fatty acid oxidation pathway control state|fatty acid oxidation (FAO, F-pathway)''']] avoiding to overestimate it?'''
:::::::- Did you know that '''malate alone might support mt-respiration''', e.g. if the [[Malic enzyme| mt-malic enzyme]] is present in [[Mitochondrial preparations| mitochondrial preparations]]? Learn more about - »'''[[Malate anaplerotic pathway control state|malate anaplerotic pathway control state]]'''«
:::::::- Did you know that '''malate alone might support mt-respiration''', if malate anaplerotic pathways [[Malic enzyme|e.g. mt-malic enzyme]] are present in [[Mitochondrial preparations| mitochondrial preparations]]? Learn more about - »'''[[Malate anaplerotic pathway control state|malate anaplerotic pathway control state]]'''«
:::::::- '''DatLab 7.4''' provides a step-by-step guide through the '''SUIT-027''' to evaluate the malate anaplerotic pathway - »'''[[SUIT-027 |SUIT-027]]'''«
:::::::- '''DatLab 7.4''' provides a step-by-step guide through the '''SUIT-027''' to evaluate the malate anaplerotic pathway - »'''[[SUIT-027 |SUIT-027]]'''«
:::::::- [[Fatty acid oxidation pathway control state|F-pathway]] can be overestimated due to anaplerotic reactions. '''SUIT-002''' is specially designed to give information on '''F-pathway in OXPHOS state avoiding FAO overestimation''' in the presence of [[Anaplerosis|anaplerotic pathways]] - »'''[[SUIT-002 |SUIT-002]]'''«
:::::::- [[Fatty acid oxidation pathway control state|F-pathway]] can be overestimated due to anaplerotic reactions. '''SUIT-002''' is specially designed to give information on '''F-pathway in OXPHOS state avoiding FAO overestimation''' in the presence of [[Anaplerosis|anaplerotic pathways]] - »'''[[SUIT-002 |SUIT-002]]'''«

Revision as of 10:21, 30 April 2020


Bioblast alert 2020

Next

MitoPedia: SUIT Do you know how to evaluate fatty acid oxidation (FAO, F-pathway) avoiding to overestimate it?
- Did you know that malate alone might support mt-respiration, if malate anaplerotic pathways e.g. mt-malic enzyme are present in mitochondrial preparations? Learn more about - »malate anaplerotic pathway control state«
- DatLab 7.4 provides a step-by-step guide through the SUIT-027 to evaluate the malate anaplerotic pathway - »SUIT-027«
- F-pathway can be overestimated due to anaplerotic reactions. SUIT-002 is specially designed to give information on F-pathway in OXPHOS state avoiding FAO overestimation in the presence of anaplerotic pathways - »SUIT-002«


MitoPedia: SUIT How can I measure ATP production in my cultured cells?
- Measuring respiration of living cells will provide you important parameters, but calling it ATP production is not correct - »SUIT protocols to measure ATP production«
- Learn more about how to measure ATP production in mitochondrial preparations with Magnesium Green - »Magnesium Green«


O2k-Publications in the MiPMap Parkinson’s Disease Awareness Month: Dominant mutation in Parkinson’s Disease causes loss of protein function in mitochondria
- from the O2k-Network »FR Angers Gueguen N« and »BE Leuven Spinazzi M«
  • Cornelissen T, Spinazzi M, Martin S, Imberechts D, Vangheluwe P, Bird M, De Strooper B, Vandenberghe W (2020) CHCHD2 harboring the Parkinson's disease-linked T61I mutation precipitates inside mitochondria and induces precipitation of wild-type CHCHD2. Hum Mol Genet [Epub ahead of print]. »Bioblast link«


O2k-brief repository Novel diurnal action of of mitochondrial stress-induced GDF15 in systemic metabolism regulation
- from the O2k-Network »DE Nuthetal Klaus S«
  • Ost M, Igual Gil C, Coleman V, Keipert S, Efstathiou S, Vidic V, Weyers M, Klaus S (2020) Muscle-derived GDF15 drives diurnal anorexia and systemic metabolic remodeling during mitochondrial stress. EMBO Rep [Epub ahead of print]. - »Bioblast link«


O2k-Network
An amazing number of new O2k-Publications from China » O2k-Network Labs China «


Gentle Science
Let's talk COVID-19 - a new culture of communication in science. - Kay Kupferschmidt in Science



MitoFit
The MitoFit Preprint Archives was created as an Open Access preprint server for mitochondrial physiology and bioenergetics - »MitoFit Preprint Archives«


O2k-Publications in the MiPMap
Zika virus infection leads to mitochondrial dysfunction
- from the O2k-Network »BR Rio de Janeiro Galina A«
  • Ledur PF, Karmirian K, Pedrosa CDSG, Souza LRQ, Assis-de-Lemos G, Martins TM, Ferreira JCCG, de Azevedo Reis GF, Silva ES, Silva D, Salerno JA, Ornelas IM, Devalle S, Madeiro da Costa RF, Goto-Silva L, Higa LM, Melo A, Tanuri A, Chimelli L, Murata MM, Garcez PP, Filippi-Chiela EC, Galina A, Borges HL, Rehen SK (2020) Zika virus infection leads to mitochondrial failure, oxidative stress and DNA damage in human iPSC-derived astrocytes. Sci Rep 10:1218. - »Bioblast link«
O2k-brief repository Could exercise protect against the effects of aging on mitochondrial function?
- from the O2k-Network »KR Busan Han J« »KR Incheon Kwak HB«
  • No MH, Heo JW, Yoo SZ, Kim CJ, Park DH, Kang JH, Seo DY, Han J, Kwak HB (2020) Effects of aging and exercise training on mitochondrial function and apoptosis in the rat heart. Pflugers Arch 472:179-93. - »Bioblast link«

On hold

MitoPedia Did you ever think that units for body mass could be an issue? » Body mass «


MitoPedia Why do we fight gas bubbles? - Dive deep into Oxygen solubility

MitoPedia alert

Next

O2k-Publications alert

Next

O2k-Open Support alert

Next

  • While in Home Office: Gnaigere (talk) 14:03, 19 March 2020 (CET) https://wiki.oroboros.at/index.php/Talk:O2k-Open_Support_alert - Many labs will move to home office mode. In line with our special DL7.4 home office offer (see Agenda today), therefore, I suggest to post in our Agenda analysis-linked O2k-Open Support cases during this time, and collect real-time operation cases for future releases.


Already added to Agenda - Next and to be added to O2k-Open support alert

  • May-18 Mo
O2k-Open Support Warning: loss of data Do you know that a humid O2k-environment and/or liquid spillage may lead to a high raw POS signal [V]?
See how to clean and dry the electrical connections of the OroboPOS-Connector.-»Cleaning the electrical connections«
  • May-25 Mo
O2k-Open Support Take care Do you know how the "switch components" approach can help you find what is causing your O2k to not reach the quality control specifications?
Do not despair if specifications given in the O2k-Manuals are not obtained. See how to troubleshoot on your own.-»Switch components approach«

Removed for correction

O2k-Open Support Warning: loss of data Do you perform the leaky chamber test after chamber assembly?
- An unnoticed leak from the chamber may affect your data, therefore we recommend performing the Leaky chamber test - especially after chamber reassembly. - »Leaky chamber test«


» https://intern.oroboros.at/index.php/Overnight_test_after_O2k-chamber_assembly

O2k-Open Support alert circulars

O2k-Open Support alert circular 2020(01): in prep

New O2k-Open Support Warning: loss of data A damaged OroboPOS-Holder may cause medium to leak from the O2k-Chamber.
See how you can check that the two blue OroboPOS-Holders are intact. - »O2k-OroboPOS-Holder«


New O2k-Open SupportTake care Calibration parameters are transferred to experimental DatLab files
Save calibration information from selected Marks while DatLab is connected to the O2k during an instrumental calibration run. Air calibration and zero oxygen calibration can be performed and saved in a single DatLab file or in separate DatLab files. - » Oxygen calibration - DatLab«
O2k-Open Support Warning: loss of data Do you perform O2k-Chamber volume calibration?
The volume calibration is essential to ensure that the chamber volume is correctly calibrated to its standard volume of 2 mL (or 0.5 mL for the O2k-sV-Module). - »O2k-Chamber MitoPedia«
O2k-Open Support Take care Do you use the correct O2 solubility factor (FM) in the ‘O2 calibration’ window in DatLab?
The O2 solubility factor varies according to the medium used and influences the air calibration. - »Oxygen solubility factor«== In preparation ==

For discussion

Here we discuss suggestions for the Oroboros Ecosystem Agenda 2020
Controlled stability of the MitoKit-CII based on HPLC and LCMS analysis: No degradation of MitoKit-CII compounds MitoKit-CII/Succinate-nv (NV118) and MitoKit-CII/Malonate-nv (NV161) for following storage conditions:
  • Proven -80 °C solid stability for more than 3 years (3 years 7 months)
  • Proven -20 °C solid stability for 1 year
  • Proven DMSO stability at room temperature for 1 week
  • Proven DMSO stability at -20 °C for 6 months (ongoing)



MitoPedia
As part of the H2020 SME NextGen-O2k project we are developing a PhotoBiology module that will allow to study the effect of different light intensities on O2 consumption and production: »PhotoBiology«
O2k-Open Support Take care Do you perform a stirrer test during oxygen calibration?
In DatLab 7.4, instrumental DL-Protocols guide you automatically towards performing the stirrer test in the process of air calibration. It shows immediately, if your OroboPOS has a satisfactory response time. Further details in MitoPedia: »Oxygen calibration - DatLab
O2k-Open Support Warning: loss of data Is the noise of the ‘O2 slope neg’ acceptable?
If you are not certain that the noise of the ‘O2 slope neg’ is within a suitable range or you want to confirm that your experimental data will not be affected, see further details: MiPNet06.03 POS-calibration-SOP

2021

Postponed events

FAT4BRAIN » FAT4BRAIN School Riga LV IOC147«
»APS2020 Chicago US«
O2k-Workshops San Diego CA US