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Difference between revisions of "Template talk:Next oroboros ecosystem agenda"

From Bioblast
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=== Next ===
=== Next ===
::'''''New'''''  [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|40px|Info: improve]] '''Do you know that you can underestimate/overestimate your respiratory fluxes? [[Steady state]] measured with high-resolution respirometry is the key to obtain correct values.''' This applies to other O2k-Applications (e.g. H<sub>2</sub>O<sub>2</sub> flux per volume).
::::::: (1) During your experiments, do you wait until the steady state is reached?
::::::: (2) Where do you set a mark?
::::::: »[[ Steady state | Guidelines for DatLab performance and data analysis:
Steady state]]«


::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] Parkinson’s Disease Awareness Month:''' Dominant mutation in Parkinson’s Disease causes loss of protein function in mitochondria'''  
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] Parkinson’s Disease Awareness Month:''' Dominant mutation in Parkinson’s Disease causes loss of protein function in mitochondria'''  
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:::: [[File:MitoPedia.jpg|60px|MitoPedia]] Why do we fight gas bubbles? - Dive deep into '''[[Oxygen solubility]]'''
:::: [[File:MitoPedia.jpg|60px|MitoPedia]] Why do we fight gas bubbles? - Dive deep into '''[[Oxygen solubility]]'''


== MitoPedia alert ==
== MitoPedia alert ==

Revision as of 11:08, 22 April 2020


Bioblast alert 2020

Next

New O2k-Open SupportInfo: improve Do you know that you can underestimate/overestimate your respiratory fluxes? Steady state measured with high-resolution respirometry is the key to obtain correct values. This applies to other O2k-Applications (e.g. H2O2 flux per volume).
(1) During your experiments, do you wait until the steady state is reached?
(2) Where do you set a mark?
» Guidelines for DatLab performance and data analysis: Steady state«
O2k-Publications in the MiPMap Parkinson’s Disease Awareness Month: Dominant mutation in Parkinson’s Disease causes loss of protein function in mitochondria
- from the O2k-Network »FR Angers Gueguen N« and »BE Leuven Spinazzi M«
  • Cornelissen T, Spinazzi M, Martin S, Imberechts D, Vangheluwe P, Bird M, De Strooper B, Vandenberghe W (2020) CHCHD2 harboring the Parkinson's disease-linked T61I mutation precipitates inside mitochondria and induces precipitation of wild-type CHCHD2. Hum Mol Genet [Epub ahead of print]. »Bioblast link«


O2k-brief repository Novel diurnal action of of mitochondrial stress-induced GDF15 in systemic metabolism regulation
- from the O2k-Network »DE Nuthetal Klaus S«
  • Ost M, Igual Gil C, Coleman V, Keipert S, Efstathiou S, Vidic V, Weyers M, Klaus S (2020) Muscle-derived GDF15 drives diurnal anorexia and systemic metabolic remodeling during mitochondrial stress. EMBO Rep [Epub ahead of print]. - »Bioblast link«


O2k-Network
An amazing number of new O2k-Publications from China » O2k-Network Labs China «


Gentle Science
Let's talk COVID-19 - a new culture of communication in science. - Kay Kupferschmidt in Science



MitoFit
The MitoFit Preprint Archives was created as an Open Access preprint server for mitochondrial physiology and bioenergetics - »MitoFit Preprint Archives«


O2k-Publications in the MiPMap
Zika virus infection leads to mitochondrial dysfunction
- from the O2k-Network »BR Rio de Janeiro Galina A«
  • Ledur PF, Karmirian K, Pedrosa CDSG, Souza LRQ, Assis-de-Lemos G, Martins TM, Ferreira JCCG, de Azevedo Reis GF, Silva ES, Silva D, Salerno JA, Ornelas IM, Devalle S, Madeiro da Costa RF, Goto-Silva L, Higa LM, Melo A, Tanuri A, Chimelli L, Murata MM, Garcez PP, Filippi-Chiela EC, Galina A, Borges HL, Rehen SK (2020) Zika virus infection leads to mitochondrial failure, oxidative stress and DNA damage in human iPSC-derived astrocytes. Sci Rep 10:1218. - »Bioblast link«
O2k-brief repository Could exercise protect against the effects of aging on mitochondrial function?
- from the O2k-Network »KR Busan Han J« »KR Incheon Kwak HB«
  • No MH, Heo JW, Yoo SZ, Kim CJ, Park DH, Kang JH, Seo DY, Han J, Kwak HB (2020) Effects of aging and exercise training on mitochondrial function and apoptosis in the rat heart. Pflugers Arch 472:179-93. - »Bioblast link«


On hold

MitoPedia Did you ever think that units for body mass could be an issue? » Body mass «


MitoPedia Why do we fight gas bubbles? - Dive deep into Oxygen solubility

MitoPedia alert

O2k-Publications alert

Next

O2k-Publications in the MiPMap The mitochondrial gas pedal: Cytosolic calcium controls extramitochondrial pyruvate generation and mitochondrial pyruvate uptake
- from the O2k-Network » FI Helsinki Jacobs HT « » DE Magdeburg Gellerich FN « and»DE Magdeburg Debska-Vielhaber G«
  • Szibor Marten, Gizatullina Zemfira, Gainutdinov Timur, Endres Thomas, Debska-Vielhaber Grazyna, Kunz Matthias, Karavasili Niki, Hallmann Kerstin, Schreiber Frank, Bamberger Alexandra, Schwarzer Michael, Doenst Torsten, Heinze Hans-Jochen, Leßmann Volkmar, Vielhaber Stefan, Kunz Wolfram S, Gellerich Frank Norbert (2020) Cytosolic, but not matrix, calcium is essential for adjustment of mitochondrial pyruvate supply. J Biol Chem [Epub ahead of print]. »Bioblast link«
O2k-Publications in the MiPMap Expression of alternative oxidase prevents reverse electron transfer formation in heart mitochondria isolated from mouse
- from the O2k-Network »FI Helsinki Jacobs HT«  »DE Magdeburg Gellerich FN« »FI Tampere Dufour E« »DE Magdeburg Debska-Vielhaber G« »DE Frankfurt Wittig I« and »UK Brighton Moore AL«
  • Szibor Marten, Gainutdinov Timur, Fernandez-Vizarra Erika, Dufour Eric, Gizatullina Zemfira, Debska-Vielhaber Grazyna, Heidler Juliana, Wittig Ilka, Viscomi Carlo, Gellerich Frank Norbert, Moore Anthony L (2019) Bioenergetic consequences from xenotopic expression of a tunicate AOX in mouse mitochondria: switch from RET and ROS to FET. Biochim Biophys Acta Bioenerg 1861:148137. »Bioblast link«
O2k-Publications in the MiPMap Mitochondria - a driving force in adipocyte differentiation
- from the O2k-Network »CZ Pilsen Kuncova J«
  • Kladnická I, Čedíková M, Kripnerová M, Dvořáková J, Kohoutová M, Tůma Z, Müllerová D, Kuncová J (2019) Mitochondrial respiration of adipocytes differentiating from human mesenchymal stem cells derived from adipose tissue. Physiol Res 68:287-96. »Bioblast link«

O2k-Open Support alert

Next

  • While in Home Office: Gnaigere (talk) 14:03, 19 March 2020 (CET) https://wiki.oroboros.at/index.php/Talk:O2k-Open_Support_alert - Many labs will move to home office mode. In line with our special DL7.4 home office offer (see Agenda today), therefore, I suggest to post in our Agenda analysis-linked O2k-Open Support cases during this time, and collect real-time operation cases for future releases.


  • Apr-27 Mo
O2k-Open SupportTake care Do you select the appropriate marks for the instrumental background correction when planning to apply them to a protocol with a high O2 regime?
To obtain an accurate O2 background correction, you must select the marks that correspond to your range of experimental O2 levels. - » O2 Background correction - troubleshooting«


  • May-04 Mo
O2k-Open SupportTake care Do you load the correct instrumental background correction accordingly to your range of experimental O2 levels?
See how to perform the instrumental background test. - » O2 background correction in DatLab« and » Instrumental oxygen background«


  • May-11 Mo
O2k-Open Support Take care Do you know how the "switch components" approach can help you find what is causing your O2k to not reach the quality control specifications?
Do not despair if specifications given in the O2k-Manuals are not obtained. See how to troubleshoot on your own.-»Switch components approach«


  • May-18 Mo
O2k-Open Support Warning: loss of data Do you know that a humid O2k-environment and/or liquid spillage may lead to a high raw POS signal [V]?
See how to clean and dry the electrical connections of the OroboPOS-Connector.-»Cleaning the electrical connections«


Removed for correction

O2k-Open Support Warning: loss of data Do you perform the leaky chamber test after chamber assembly?
- An unnoticed leak from the chamber may affect your data, therefore we recommend performing the Leaky chamber test - especially after chamber reassembly. - »Leaky chamber test«


» https://intern.oroboros.at/index.php/Overnight_test_after_O2k-chamber_assembly

O2k-Open Support alert circulars

O2k-Open Support alert circular 2020(01): in prep

New O2k-Open Support Warning: loss of data A damaged OroboPOS-Holder may cause medium to leak from the O2k-Chamber.
See how you can check that the two blue OroboPOS-Holders are intact. - »O2k-OroboPOS-Holder«


New O2k-Open SupportTake care Calibration parameters are transferred to experimental DatLab files
Save calibration information from selected Marks while DatLab is connected to the O2k during an instrumental calibration run. Air calibration and zero oxygen calibration can be performed and saved in a single DatLab file or in separate DatLab files. - » Oxygen calibration - DatLab«
O2k-Open Support Warning: loss of data Do you perform O2k-Chamber volume calibration?
The volume calibration is essential to ensure that the chamber volume is correctly calibrated to its standard volume of 2 mL (or 0.5 mL for the O2k-sV-Module). - »O2k-Chamber MitoPedia«
O2k-Open Support Take care Do you use the correct O2 solubility factor (FM) in the ‘O2 calibration’ window in DatLab?
The O2 solubility factor varies according to the medium used and influences the air calibration. - »Oxygen solubility factor«== In preparation ==

For discussion

Here we discuss suggestions for the Oroboros Ecosystem Agenda 2020
MitoPedia
As part of the H2020 SME NextGen-O2k project we are developing a PhotoBiology module that will allow to study the effect of different light intensities on O2 consumption and production: »PhotoBiology«
O2k-Open Support Take care Do you perform a stirrer test during oxygen calibration?
In DatLab 7.4, instrumental DL-Protocols guide you automatically towards performing the stirrer test in the process of air calibration. It shows immediately, if your OroboPOS has a satisfactory response time. Further details in MitoPedia: »Oxygen calibration - DatLab
O2k-Open Support Warning: loss of data Is the noise of the ‘O2 slope neg’ acceptable?
If you are not certain that the noise of the ‘O2 slope neg’ is within a suitable range or you want to confirm that your experimental data will not be affected, see further details: MiPNet06.03 POS-calibration-SOP

2021

Postponed events

FAT4BRAIN » FAT4BRAIN School Riga LV IOC147«
»APS2020 Chicago US«
O2k-Workshops San Diego CA US