Difference between revisions of "Template:SUIT text D062"
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SUIT-003 O2 ce D062 protocol is the control of the [[SUIT-003 O2 ce D050]] adding the [[Carrier control titrations|carrier]] DMSO in [[ROUTINE]] respiration instead of the plasma membrane-permeable succinate ([[MitoKit-CII/Succinate-nv]]). Moreover,the SUIT-003 O2 ce D062 protocol allows to evaluate the [[coupling control state |coupling control]] of living cells. Respiratory capacities are tested in a sequence of coupling states: [[ROUTINE]] and [[Electron transfer pathway]]. Optionally, to study [[LEAK respiration]], the [[phosphorylation system]] might be inhibited by [[oligomycin]]. The final concentration of [[oligomycin]] has to be carefully optimized for various cell types, to minimize the inhibitory effect on the [[Electron transfer | SUIT-003 O2 ce D062 protocol is the control of the [[SUIT-003 O2 ce D050]] adding the [[Carrier control titrations|carrier]] DMSO in [[ROUTINE]] respiration instead of the plasma membrane-permeable succinate ([[MitoKit-CII/Succinate-nv]]). Moreover,the SUIT-003 O2 ce D062 protocol allows to evaluate the [[coupling control state |coupling control]] of living cells. Respiratory capacities are tested in a sequence of coupling states: [[ROUTINE]] and [[Electron transfer pathway]]. Optionally, to study [[LEAK respiration]], the [[phosphorylation system]] might be inhibited by [[oligomycin]]. The final concentration of [[oligomycin]] has to be carefully optimized for various cell types, to minimize the inhibitory effect on the [[Electron transfer pathway|electron transfer system]] which would lead to an underestimation of [[ET-capacity]]. | ||
Revision as of 14:40, 8 June 2020
SUIT-003 O2 ce D062 protocol is the control of the SUIT-003 O2 ce D050 adding the carrier DMSO in ROUTINE respiration instead of the plasma membrane-permeable succinate (MitoKit-CII/Succinate-nv). Moreover,the SUIT-003 O2 ce D062 protocol allows to evaluate the coupling control of living cells. Respiratory capacities are tested in a sequence of coupling states: ROUTINE and Electron transfer pathway. Optionally, to study LEAK respiration, the phosphorylation system might be inhibited by oligomycin. The final concentration of oligomycin has to be carefully optimized for various cell types, to minimize the inhibitory effect on the electron transfer system which would lead to an underestimation of ET-capacity.
