Difference between revisions of "Template:SUIT text D024"
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The SUIT-011 O2 pfi D024 protocol is designed to study physiologically relevant maximum mitochondrial respiratory capacity ([[Oxidative phosphorylation|OXPHOS]] with [[NS-pathway control state|NS substrates]]) and coupling/pathway control states. SUIT-011 gives information of the linear coupling control ([[LEAK | The SUIT-011 O2 pfi D024 protocol is designed to study physiologically relevant maximum mitochondrial respiratory capacity ([[Oxidative phosphorylation|OXPHOS]] with [[NS-pathway control state|NS substrates]]) and coupling/pathway control states. SUIT-011 gives information of the linear coupling control ([[LEAK respiration|''L'']]-[[Oxidative phosphorylation| ''P'']]) with NADH linked-substrates ([[GM-pathway control state|GM]]). GM and PM yield practically identical fluxes in human skeletal muscle fibers. However, PM is the superior alternative to GM, since the fractions of the N-pathway is lower and of the S-pathway is higher with GM compared to PM. PM, therefore, yields a more sensitive assay for the diagnosis of injuries in the N-linked pathway (compare [[SUIT-001]] and [[SUIT-004]]). Moreover, SUIT-011 allows the evaluation of the coupling control state ([[Oxidative phosphorylation|''P'']]-[[ET capacity| ''E'']]) with NADH and succinate linked-substrates ([[NS-pathway control state|NS]]) and the pathway control in OXPHOS ([[NS-pathway control state|NS]]) and ET state ([[NS-pathway control state|NS]] and [[Succinate pathway control state|S]]). SUIT-011 can be extended with the CIV assay module. | ||
Latest revision as of 16:12, 8 June 2020
The SUIT-011 O2 pfi D024 protocol is designed to study physiologically relevant maximum mitochondrial respiratory capacity (OXPHOS with NS substrates) and coupling/pathway control states. SUIT-011 gives information of the linear coupling control (L- P) with NADH linked-substrates (GM). GM and PM yield practically identical fluxes in human skeletal muscle fibers. However, PM is the superior alternative to GM, since the fractions of the N-pathway is lower and of the S-pathway is higher with GM compared to PM. PM, therefore, yields a more sensitive assay for the diagnosis of injuries in the N-linked pathway (compare SUIT-001 and SUIT-004). Moreover, SUIT-011 allows the evaluation of the coupling control state (P- E) with NADH and succinate linked-substrates (NS) and the pathway control in OXPHOS (NS) and ET state (NS and S). SUIT-011 can be extended with the CIV assay module.
