Difference between revisions of "Template:SUIT text D009"
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The SUIT-003 O2 ce D009 (CCP-ce) is designed to study [[coupling control state |coupling control]] of living cells. Respiratory capacities are tested in a sequence of coupling states: [[ROUTINE]], [[LEAK]] and [[Electron transfer pathway]]. To study [[LEAK | The SUIT-003 O2 ce D009 (CCP-ce) is designed to study [[coupling control state |coupling control]] of living cells. Respiratory capacities are tested in a sequence of coupling states: [[ROUTINE]], [[LEAK]] and [[Electron transfer pathway]]. To study [[LEAK respiration]], the [[phosphorylation system]] is inhibited by [[oligomycin]]. The final concentration of [[oligomycin]] has to be carefully optimized for various cell types, to minimize the inhibitory effect on the [[Electron transfer-pathway|electron transfer system]] which would lead to an underestimation of [[ET-capacity]]. | ||
Revision as of 10:37, 8 June 2020
The SUIT-003 O2 ce D009 (CCP-ce) is designed to study coupling control of living cells. Respiratory capacities are tested in a sequence of coupling states: ROUTINE, LEAK and Electron transfer pathway. To study LEAK respiration, the phosphorylation system is inhibited by oligomycin. The final concentration of oligomycin has to be carefully optimized for various cell types, to minimize the inhibitory effect on the electron transfer system which would lead to an underestimation of ET-capacity.
