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Template:SUIT-006 O2 D051

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MitoPedia: SUIT

Steps and respiratory states

1X;2D;2c;3Omy;4U;5Ama.png


Step State Pathway Q-junction Comment - Events (E) and Marks (M)


1X XL(n) 1X
  • Electron-transfer-pathway state will depend on the substrate/inhibitor combinations added to fuel the mitochondrial respiration
  • Non-phosphorylating resting state (LEAK state); LEAK respiration L(n) in the absence of ADP, ATP, AMP (no adenylates).
2D XP 1X;2D
2c XcP 1X;2D;2c
  • Electron-transfer-pathway state will depend on the substrate/inhibitor combinations added to fuel the mitochondrial respiration
  • OXPHOS capacity P (with saturating [ADP]), active OXPHOS state.
  • Addition of cytochrome c yields a test for integrity of the mtOM (cytochrome c control efficiency). Stimulation by added cytochrome c would indicate an injury of the mtOM and limitation of respiration in the preceding state without added c due to loss of cytochrome c. Typically, cytochrome c is added immediately after the earliest ADP-activation step (OXPHOS capacity P with saturating [ADP]).
3(Omy) XL(Omy) 1X;2D;2c;3(Omy)
  • Electron-transfer-pathway state will depend on the substrate/inhibitor combinations added to fuel the mitochondrial respiration
  • Non-phosphorylating resting state (LEAK state); LEAK-respiration, L(Omy), after blocking the ATP synthase with oligomycin.
  • The addition of oligomycin is optional depending on the experimental question to resolve. If it is known that Ln has the same values for LOmy for a kind of sample and substrates, this step may be skipped. If the carrier of oligomycin (EtOH) is used instead, it is possible to evaluate whether it affects respiration. In these cases, LEAK state is not reached again.
4U XE 1X;2D;2c;3(Omy);4U
5* ROX 1X;2D;2c;3(Omy);4U;5*
  • (*) Rox obtaining after inhibiting the CIII (e.g. antimycin A, myxothiazol).
  • Rox is the residual oxygen consumption in the ROX state, due to oxidative side reactions, estimated after addition of antimycin A (inhibitor of CIII). Rox is subtracted from oxygen flux as a baseline for all respiratory states, to obtain mitochondrial respiration (mt).


Step Respiratory state Pathway control ET-Complex Comment
## AsTm AsTmE CIV CIV
## Azd CHB


Questions.jpg


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