Difference between revisions of "Talk:SUIT protocol library"
From Bioblast
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* Protocol 1: G + M + D + H202 (wait 20 min) + Ctl + c + P + D + S + Omy + FCCP+ Rot + Mna + Ama | * Protocol 1: G + M + D + H202 (wait 20 min) + Ctl + c + P + D + S + Omy + FCCP+ Rot + Mna + Ama | ||
To reduce the number of protocols, I suggest to skip Protocol 1, and try Protocol 2 only (Protocol 1 without Omy was applied before, the results will serve as a basis to describe the general substrate control pattern. | To reduce the number of protocols, I suggest to skip Protocol 1, and try Protocol 2 only (Protocol 1 without Omy was applied before, the results will serve as a basis to describe the general substrate control pattern). | ||
* Protocol 2: G + M + D + H202 (wait 20 min) + Ctl + c + P + D + S * Rot + Omy + FCCP + + Rot + Mna + Ama | * Protocol 2: G + M + D + H202 (wait 20 min) + Ctl + c + P + D + S * Rot + Omy + FCCP + + Rot + Mna + Ama | ||
''Correction (EG, 2011-10-11)'': | ''Correction (EG, 2011-10-11)'': | ||
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: see the following protocol for the H202 - oxidative stress - treatment: [[DSL_intern#Protocol:_2011-10-06]] | : see the following protocol for the H202 - oxidative stress - treatment: [[DSL_intern#Protocol:_2011-10-06]] | ||
: '''Rational:''' calculation of P/E is not possible, but the effect of Omy is of interest | : '''Rational:''' calculation of P/E is not possible, but the effect of Omy is of interest | ||
=== Mouse brain - Shredder === | === Mouse brain - Shredder === | ||
Revision as of 17:59, 11 October 2011
Future Projects/Protocols
Fish - Oxidative stress
- Protocol 1: G + M + D + H202 (wait 20 min) + Ctl + c + P + D + S + Omy + FCCP+ Rot + Mna + Ama
To reduce the number of protocols, I suggest to skip Protocol 1, and try Protocol 2 only (Protocol 1 without Omy was applied before, the results will serve as a basis to describe the general substrate control pattern).
- Protocol 2: G + M + D + H202 (wait 20 min) + Ctl + c + P + D + S * Rot + Omy + FCCP + + Rot + Mna + Ama
Correction (EG, 2011-10-11):
- Protocol 2: G + M + D + H202 (wait 20 min) + Ctl + c + P + D + S + D + Rot + Omy + FCCP + Mna + Ama
- see the following protocol for the H202 - oxidative stress - treatment: DSL_intern#Protocol:_2011-10-06
- Rational: calculation of P/E is not possible, but the effect of Omy is of interest
Mouse brain - Shredder
- Idea by Erich, 2011-10-07
- Method: Shredder
- Rational: test these different protocols to see the effect of cytochrome c and Oligomycin at different positions in the protocol
- Protocol 1: PGM + c + D-titration + Omy + F-titration + Rot + Mna + Ama
- Protocol 2: PGM + D-titration + c + Omy + F-titration + Rot + Mna + Ama
- Protocol 3: PGM + D-titration + Omy + c + F-titration + Rot + Mna + Ama
