https://wiki.oroboros.at/index.php?title=Talk:SUIT-016&feed=atom&action=history
Talk:SUIT-016 - Revision history
2024-03-29T13:40:06Z
Revision history for this page on the wiki
MediaWiki 1.36.1
https://wiki.oroboros.at/index.php?title=Talk:SUIT-016&diff=202948&oldid=prev
Beno Marija at 12:39, 3 June 2020
2020-06-03T12:39:00Z
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td>
<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 12:39, 3 June 2020</td>
</tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l21">Line 21:</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>| [[F]]</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>| [[F]]</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>| CETF</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>| CETF</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>| [[OXPHOS coupling efficiency]] in state F.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>| [[OXPHOS<ins style="font-weight: bold; text-decoration: none;">-</ins>coupling efficiency]] in state F.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|-</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|-</div></td></tr>
<tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l42">Line 42:</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>| [[S]]</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>| [[S]]</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>| CII</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>| CII</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>| The ''P/E'' ratio is not obtained in the FNS state, with preference given to the next steps for OXPHOS coupling efficiency. A succinate concentration of >10 mM may be required for saturating S<sub>''P''</sub> capacity.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>| The ''P/E'' ratio is not obtained in the FNS state, with preference given to the next steps for OXPHOS<ins style="font-weight: bold; text-decoration: none;">-</ins>coupling efficiency. A succinate concentration of >10 mM may be required for saturating S<sub>''P''</sub> capacity.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|-</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|-</div></td></tr>
<tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l49">Line 49:</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>| [[S]]</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>| [[S]]</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>| CII</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>| CII</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>| [[OXPHOS coupling efficiency]] in state S, comparable to state F.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>| [[OXPHOS<ins style="font-weight: bold; text-decoration: none;">-</ins>coupling efficiency]] in state S, comparable to state F.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|-</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|-</div></td></tr>
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Beno Marija
https://wiki.oroboros.at/index.php?title=Talk:SUIT-016&diff=169537&oldid=prev
Doerrier Carolina: Doerrier Carolina moved page Talk:1OctM;2D;3G;4S;5Rot;6Omy;7U- to Talk:SUIT-016
2019-01-15T09:47:58Z
<p>Doerrier Carolina moved page <a href="/index.php/Talk:1OctM;2D;3G;4S;5Rot;6Omy;7U-" class="mw-redirect" title="Talk:1OctM;2D;3G;4S;5Rot;6Omy;7U-">Talk:1OctM;2D;3G;4S;5Rot;6Omy;7U-</a> to <a href="/index.php/Talk:SUIT-016" title="Talk:SUIT-016">Talk:SUIT-016</a></p>
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<td colspan="1" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td>
<td colspan="1" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 09:47, 15 January 2019</td>
</tr><tr><td colspan="2" class="diff-notice" lang="en"><div class="mw-diff-empty">(No difference)</div>
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Doerrier Carolina
https://wiki.oroboros.at/index.php?title=Talk:SUIT-016&diff=169531&oldid=prev
Doerrier Carolina: Created page with "::: '''SUIT states:''' 1-2OctM(LP);3GMOct;4GMSOct;5-7S(PLEc);8ROX {| class="wikitable" border="1" |- ! Step ! Respiratory state ! Pathway control ! Pathwa..."
2019-01-15T09:45:57Z
<p>Created page with "::: '''SUIT states:''' 1-2<a href="/index.php/OctM" class="mw-redirect" title="OctM">OctM</a>(LP);3<a href="/index.php?title=GMOct&action=edit&redlink=1" class="new" title="GMOct (page does not exist)">GMOct</a>;4<a href="/index.php?title=GMSOct&action=edit&redlink=1" class="new" title="GMSOct (page does not exist)">GMSOct</a>;5-7<a href="/index.php/S" class="mw-redirect" title="S">S</a>(PLEc);8<a href="/index.php/ROX" class="mw-redirect" title="ROX">ROX</a> {| class="wikitable" border="1" |- ! Step ! Respiratory state ! Pathway control ! Pathwa..."</p>
<p><b>New page</b></p><div>::: '''SUIT states:''' 1-2[[OctM]](LP);3[[GMOct]];4[[GMSOct]];5-7[[S]](PLEc);8[[ROX]]<br />
<br />
{| class="wikitable" border="1"<br />
|-<br />
! Step<br />
! Respiratory state<br />
! Pathway control<br />
! Pathway to Q<br />
! Comment<br />
<br />
|-<br />
| 1OctM<br />
| [[OctM]](L)<br />
| [[F]]<br />
| CETF<br />
| <br />
<br />
|-<br />
| 2D<br />
| [[OctM]](P)<br />
| [[F]]<br />
| CETF<br />
| [[OXPHOS coupling efficiency]] in state F.<br />
<br />
|-<br />
| 3G<br />
| [[GMOct]](P)<br />
| [[FN]]<br />
| FAO&CI<br />
| F/N ratio or (N-F)/N flux control factor of OXPHOS capacity.<br />
<br />
|-<br />
| 4S<br />
| [[GMSOct]](P)<br />
| [[FNS]]<br />
| FAO&CI&II<br />
| Additive effect of the NS pathway combination, compared to the sum of N+S pathway fluxes measured separately, in the OXPHOS state and the presence of F.<br />
<br />
|-<br />
| 5Rot<br />
| [[S]](P)<br />
| [[S]]<br />
| CII<br />
| The ''P/E'' ratio is not obtained in the FNS state, with preference given to the next steps for OXPHOS coupling efficiency. A succinate concentration of >10 mM may be required for saturating S<sub>''P''</sub> capacity.<br />
<br />
|-<br />
| 6Omy<br />
| [[S]](L)<br />
| [[S]]<br />
| CII<br />
| [[OXPHOS coupling efficiency]] in state S, comparable to state F.<br />
<br />
|-<br />
| 7U<br />
| [[S]](E)<br />
| [[S]]<br />
| CII<br />
| The complete ET capacity in state S may not be obtained after oligomycin. If apparent P/E>1.0, this is corrected to 1.0.<br />
<br />
|-<br />
| 7c<br />
| [[S]](E)<br />
| [[S]]<br />
| CII<br />
| If [[cytochrome c control factor]]s = 0.0, the cytochrome ''c'' test conducted at this very late ET-pathway state provides an additional indication of stability of the mt-preparation during the experimental assay. Application of the cytochrome ''c'' test earlier in the protocol ([[SUIT-011]]: 1GM;2D;2c;3S; ..) is preferable, if [[cytochrome c control factor]]s > 0.0 may be observed occasionally or regulary. If such cytochrome ''c'' effects are not exclusion criteria, then OXPHOS states with all substrate combinations can be compared in the presence of cytochrome ''c''.<br />
<br />
|-<br />
| 8Ama<br />
|<br />
| [[ROX]]<br />
| [[ROX]]<br />
| ROX may be lower in substrate states earlier in the SUIT protocol. Therefore, this ROX measurement is frequently taken as a methodological control rather than as the basis of ROX correction of mitochondrial respiration (mt).<br />
<br />
|}</div>
Doerrier Carolina