Talk:Pyruvate
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Former Instructions
These instructions were given until 2020-03-08. They were updated because the volume of the solute was not taken into account. Therefore the concentration oft the stock solution was lower than intended.
- Preparation of 2 M stock solution (dissolved in H2O) for use in 2-mL O2k-chamber:
- Prepare fresh everyday.
- Weigh 44 mg of pyruvic acid directly into a 0.5 mL Eppendorf tube.
- Add 0.2 mL H2O.
- Adjust pH to 7.0.
- Preparation of 2.5 M stock solution (dissolved in H2O) for use in 0.5-mL O2k-chamber:
- Prepare fresh everyday.
- Weigh 55 mg of pyruvic acid directly into a 0.5 mL Eppendorf tube.
- Add 0.2 mL H2O.
- Adjust pH to 7.0.
