Difference between revisions of "Talk:Pyruvate"

Latest revision as of 13:15, 8 March 2021

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Pyruvate has been accessed more than

10,000 times (2019-08-07)
5,000 times (2016-03-21)

Former Instructions

These instructions were given until 2020-03-08. They were updated because the volume of the solute was not taken into account. Therefore the concentration oft the stock solution was lower than intended.

Preparation of 2 M stock solution (dissolved in H₂O) for use in 2-mL O2k-chamber:

Prepare fresh everyday.
Weigh 44 mg of pyruvic acid directly into a 0.5 mL Eppendorf tube.
Add 0.2 mL H₂O.
Adjust pH to 7.0.

Preparation of 2.5 M stock solution (dissolved in H₂O) for use in 0.5-mL O2k-chamber:

Prepare fresh everyday.
Weigh 55 mg of pyruvic acid directly into a 0.5 mL Eppendorf tube.
Add 0.2 mL H₂O.
Adjust pH to 7.0.

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 ::::*  10,000 times (2019-08-07)
 ::::*  5,000 times (2016-03-21)
+== Former Instructions ==
-== Application in [[HRR]] (updated instructions) ==
+These instructions were given until 2020-03-08. They were updated because the volume of the solute was not taken into account. Therefore the concentration oft the stock solution was lower than intended.
-::: '''P: Pyruvate''' (pyruvic acid, sodium salt, C<sub>3</sub>H<sub>3</sub>O<sub>3</sub>Na); Sigma P 2256, 25 g, store at 4 °C; M = 110.0 g·mol<sup>-1</sup>
+:::: '''Preparation of 2 M stock solution''' (dissolved in H<sub>2</sub>O)  for use in '''2-mL O2k-chamber''':
-:::: It is possible to weigh the powder beforehand in the Eppendorf-type tubes and store these tubes at 4 °C, to be diluted only on the day of use.
-:::: 2021-02: The preparation instructions were updated to take the volume of the solute (P) into account (see: [[User:Spitzer_Gudrun/Volume_of_the_solute]]). The concentrations prepared following the former instructions (see Discussion section) are sufficiently high for SUIT protocol titrations.
-:::: '''Preparation of 2 M stock solution''' (200 µL, dissolved in H<sub>2</sub>O)  for use in '''2-mL O2k-chamber''':
 ::::# Prepare fresh everyday.
 ::::# Weigh 44 mg of pyruvic acid directly into a 0.5 mL Eppendorf tube.
-::::# Add 180 µL H<sub>2</sub>O.
+::::# Add 0.2 mL H<sub>2</sub>O.
 ::::# Adjust pH to 7.0.
-:::» '''O2k manual titrations'''  [[MiPNet09.12 O2k-Titrations]]
+:::: '''Preparation of 2.5 M stock solution''' (dissolved in H<sub>2</sub>O)  for use in '''0.5-mL O2k-chamber''':
-::::* Titration volume ('''2-mL O2k-chamber'''): 5 µL using a 25 µL Hamilton syringe.
-::::* Final concentration: 5 mM.
-:::: '''Preparation of 2.5 M stock solution''' (200 µL, dissolved in H<sub>2</sub>O)  for use in '''0.5-mL O2k-chamber''':
 ::::# Prepare fresh everyday.
 ::::# Weigh 55 mg of pyruvic acid directly into a 0.5 mL Eppendorf tube.
-::::# Add 175 µL H<sub>2</sub>O.
+::::# Add 0.2 mL H<sub>2</sub>O.
 ::::# Adjust pH to 7.0.
-:::» '''O2k manual titrations'''  [[MiPNet09.12 O2k-Titrations]]
-::::* Titration volume ('''0.5-mL O2k-chamber'''): 1 µL using a 10 µL Hamilton syringe.
-::::* Final concentration: 5 mM.