Difference between revisions of "Talk:Pyruvate"
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(added former preparation instructions that did not take the volume of the solvent into account) ย |
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== Popular Bioblast page == | == Popular Bioblast page == | ||
:::: [[Pyruvate]] has been accessed more than ย | :::: [[Pyruvate]] has been accessed more than ย | ||
::::*ย 10,000 times (2019-08-07) | |||
::::*ย 5,000 times (2016-03-21) | ::::*ย 5,000 times (2016-03-21) | ||
== Former Instructions == | |||
These instructions were given until 2020-03-08. They were updated because the volume of the solute was not taken into account. Therefore the concentration oft the stock solution was lower than intended. | |||
:::: '''Preparation of 2 M stock solution''' (dissolved in H<sub>2</sub>O)ย for use in '''2-mL O2k-chamber''': | |||
::::# Prepare fresh everyday. | |||
::::# Weigh 44 mg of pyruvic acid directly into a 0.5 mL Eppendorf tube. | |||
::::# Add 0.2 mL H<sub>2</sub>O. | |||
::::# Adjust pH to 7.0. | |||
:::: '''Preparation of 2.5 M stock solution''' (dissolved in H<sub>2</sub>O)ย for use in '''0.5-mL O2k-chamber''': | |||
::::# Prepare fresh everyday. | |||
::::# Weigh 55 mg of pyruvic acid directly into a 0.5 mL Eppendorf tube. | |||
::::# Add 0.2 mL H<sub>2</sub>O. | |||
::::# Adjust pH to 7.0. |
Latest revision as of 13:15, 8 March 2021
Popular Bioblast page
- Pyruvate has been accessed more than
- 10,000 times (2019-08-07)
- 5,000 times (2016-03-21)
- Pyruvate has been accessed more than
Former Instructions
These instructions were given until 2020-03-08. They were updated because the volume of the solute was not taken into account. Therefore the concentration oft the stock solution was lower than intended.
- Preparation of 2 M stock solution (dissolved in H2O) for use in 2-mL O2k-chamber:
- Prepare fresh everyday.
- Weigh 44 mg of pyruvic acid directly into a 0.5 mL Eppendorf tube.
- Add 0.2 mL H2O.
- Adjust pH to 7.0.
- Preparation of 2.5 M stock solution (dissolved in H2O) for use in 0.5-mL O2k-chamber:
- Prepare fresh everyday.
- Weigh 55 mg of pyruvic acid directly into a 0.5 mL Eppendorf tube.
- Add 0.2 mL H2O.
- Adjust pH to 7.0.