Talk:Pyruvate

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Application in HRR (updated instructions)

P: Pyruvate (pyruvic acid, sodium salt, C₃H₃O₃Na); Sigma P 2256, 25 g, store at 4 °C; M = 110.0 g·mol^-1

It is possible to weigh the powder beforehand in the Eppendorf-type tubes and store these tubes at 4 °C, to be diluted only on the day of use.

2021-02: The preparation instructions were updated to take the volume of the solute (P) into account. The concentrations prepared following the former instructions (see Discussion section) are sufficiently high for SUIT protocol titrations.

Preparation of 2 M stock solution (200 µL, dissolved in H₂O) for use in 2-mL O2k-chamber:

1. Prepare fresh everyday.
2. Weigh 44 mg of pyruvic acid directly into a 0.5 mL Eppendorf tube.
3. Add 180 µL H₂O.
4. Adjust pH to 7.0.

» O2k manual titrations MiPNet09.12 O2k-Titrations

• Titration volume (2-mL O2k-chamber): 5 µL using a 25 µL Hamilton syringe.
• Final concentration: 5 mM.

Preparation of 2.5 M stock solution (200 µL, dissolved in H₂O) for use in 0.5-mL O2k-chamber:

1. Prepare fresh everyday.
2. Weigh 55 mg of pyruvic acid directly into a 0.5 mL Eppendorf tube.
3. Add 175 µL H₂O.
4. Adjust pH to 7.0.

» O2k manual titrations MiPNet09.12 O2k-Titrations

• Titration volume (0.5-mL O2k-chamber): 1 µL using a 10 µL Hamilton syringe.
• Final concentration: 5 mM.