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| =Titration and settings=
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| * 10 µl AmR - 5 µM final conc. in chamber
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| * 4µl HRP (500U/ml) - 1 U/ml final conc. in chamber* light intensity of LEDs: 1 mA ( DatLab: Amp polaristion voltage 100 mV) / Gain 1000
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| * H<sub>2</sub>O<sub>2</sub> calibration with a''' 15.8 µM H<sub>2</sub>O<sub>2</sub>''' solution - '''2012-06-20'''
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| :: '''commercial stock:''' 880 mM
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| :: dilution 1 (1:166.67): 150 µl in 25 ml H<sub>2</sub>O = 5.28 mM
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| :: dilution 2 (1:333.33): 150 µl in 50 ml H<sub>2</sub>O + 500 µl 1 mM HCl = '''15.8 µM'''
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| :: → '''calibration:''' 2 * 5 µl in 2 ml chamber = 2* 0.0395 µM = total 0.079 µM (= 79 nM)
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| * new H<sub>2</sub>O<sub>2</sub> calibration with a '''28.16 µM''' H<sub>2</sub>O<sub>2</sub> solution, valid from from '''2012-06-22''':
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| :: '''commercial stock:''' 880 mM
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| :: dilution 1 (1:125): 200 µl in 25 ml H<sub>2</sub>O = 7.04 mM
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| :: dilution 2 (1:250): 200 µl in 50 ml H<sub>2</sub>O + 500 µl 1 mM HCl = '''28.16 µM'''
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| :: → '''calibration''': 2 * 5 µl in 2 ml chamber = 2* 0.0704 µM = total 0.1408 µM (= 140.8 nM)
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