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Difference between revisions of "Talk:Komlodi 2021 MitoFit Q"

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== Versions ==
{{Publication
::::Β» [[File:Komlodi 2021 MitoFit Q.pdf]]
|title=Komlodi T, Cardoso LHD, Doerrier C, Gnaiger E (2021) Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria. MitoFit Preprints 2021.2. [[doi:10.26124/mitofit:2021-0002]]
|info=[[File:MitoFit Preprints pdf.png|left|160px|link=https://www.mitofit.org/images/Komlodi_2021_MitoFit_Q.pdf |MitoFit pdf]] [https://www.mitofit.org/images/Komlodi_2021_MitoFit_Q.pdf Simultaneous measurement of respiration and redox state of coenzyme Q in isolated mitochondria]
|authors=Komlodi Timea, Cardoso Luiza HD, Doerrier Carolina, Gnaiger Erich
|year=2021-02-15
|journal=MitoFit Prep
|abstract= Redox states of mitochondrial coenzyme Q (mtCoQ or Q) reflect the balance between (''1'') reducing capacities of electron flow from fuel substrates converging at the Q-junction, (''2'') oxidative capacities downstream of Q to oxygen, and (''3'') the load on the OXPHOS system utilizing or dissipating the protonmotive force. A three-electrode sensor (Rich 1988; Moore et al 1988) was implemented into the NextGen-O2k to monitor the Q redox state continuously and simultaneously with oxygen consumption. The Q-Module was optimized for high signal-to-noise ratio and minimum oxygen diffusion. CoQ2 is added as a redox probe equilibrating with Q at Complexes CI, CII and CIII and the detecting electrode. Q-sensors are poised with the CoQ2 redox peak potentials determined by cyclic voltammetry, which provides quality control of the Q-sensor and reveals chemical interferences.
The Q redox state and oxygen consumption were measured simultaneously in isolated mitochondria. A coupling-control protocol was applied to analyze LEAK, OXPHOS, and electron transfer capacities (''L'', ''P'', and ''E'', respectively) in the succinate-pathway. In a second pathway-control protocol, NADH- and succinate-linked pathways (N and S) converge at the Q-junction. mtCoQ was more oxidized when O2 flux was stimulated in coupling-control states with load increasing from L to P and E. In contrast, mtCoQ was more reduced when O2 flux was stimulated with electron input capacities increasing from N-, S- to NS-pathway-control states. N- and S- pathway capacities were not completely additive, thus confirming partial pool behavior of Q as proposed in the plasticity model of supercomplex organization.
|keywords=Q-junction, mitochondria, oxygen consumption, Q redox state, three-electrode system, cyclic voltammetry, harmonized SUIT protocols, high-resolution respirometry, coupling control, pathway control, NS-pathway, additivity
|editor=
|mipnetlab=AT Innsbruck Oroboros
}}
ORC'''ID''': [[File:ORCID.png|20px|link=https://orcid.org/0000-0001-9876-1411]] Komlodi Timea, [[File:ORCID.png|20px|link=https://orcid.org/0000-0001-6392-9229]] Cardoso Luiza HD, [[File:ORCID.png|20px|link=https://orcid.org/0000-0002-7969-6762]] Doerrier Carolina, [[File:ORCID.png|20px|link=https://orcid.org/0000-0003-3647-5895]] Gnaiger Erich

Revision as of 19:26, 18 February 2021