Difference between revisions of "TIP2k syringe blocked"

Latest revision as of 13:05, 8 November 2019

TIP2k syringe blocked

MitoPedia O2k and high-resolution respirometry: O2k-Open Support

Description

When the TIP2k syringe is blocked, it must not be used with the TIP2k, and specific cleaning instructions should be followed.

Reference: MiPNet12.10 TIP2k-manual.pdf

Start immediately with extensive cleaning procedures.
Suitable solvents for extensive cleaning procedures depend on the substances used. Chloroform was successful for some fatty acids.
An ultrasonic bath may be helpful in cleaning partially blocked syringes.

Once a needle is totally blocked it is usually impossible (with exceptions) to clean it.

@@ Line 1: / Line 1: @@
-==Problem==
+{{Technical support}}
-*TIP syringe is harder to operate than usually.
+__TOC__
+{{MitoPedia without banner
+|abbr=
+|description=When the '''TIP2k syringe is blocked''', it must not be used with the TIP2k, and specific cleaning instructions should be followed.
+|info=[https://wiki.oroboros.at/images/f/f5/MiPNet12.10_TIP2k-Manual.pdf MiPNet12.10 TIP2k-manual.pdf]
+}}
+:::# Start immediately with extensive cleaning procedures.
+:::# Suitable solvents for extensive cleaning procedures depend on the substances used. Chloroform was successful for some fatty acids.
+:::# An ultrasonic bath may be helpful in cleaning partially blocked syringes.
-==Solution==
+::::* Once a needle is totally blocked it is usually impossible (with exceptions) to clean it.
-Start immediately with extensive cleaning procedures [http://www.oroboros.at/index.php?id=o2k-tip2k-manual MiPNet12.10]. Once a needle is totally blocked it is usually impossible (with exceptions) to clean it.
-*Suitable solvents for extensive cleaning procedures depend on the substances used.
-*Chloroform was successful for some fatty acids. An ultrasonic bath may be helpful in cleaning partially blocked syringes.
-==Reason and Prevention==
-#To prevent washing out effect when the needle is inserted into the chamber the inner diameter of the TIP needle has to be very small. This means that the needle is easily blocked by small particles either precipitated from the solution or introduced with the solution.
-#While a TIP syringe has to be considered a consumable, it is possible to lengthen its lifetime by carefully following the procedures suggested in the TIP-2k Manual [http://www.oroboros.at/index.php?id=o2k-tip2k-manual MiPNet12.10].
-::*The main way to prevent clogging of needles is to follow rigorously the cleaning procedure outlined in [http://www.oroboros.at/index.php?id=o2k-tip2k-manual MiPNet12.10] after each usage.
-::*Additionally, it has to be taken care that no particles are suspended in the used solutions.
-::*For not very well soluble substances, filtering of the solution may be necessary. Small particles can also be introduced by laboratory activities, e.g. from paper towels or from powdered gloves.
-{{#set: Technical service=TIP2k|Technical service=Syringe}}
-__SHOWFACTBOX__
-{{Troubleshooting}}