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== Application in [[HRR]] ==
::: '''SOD: Superoxide dismutase from bovine liver''', Sigma S8160, 15 KU, store at -20°C; MW = 32.000,00 g/mol
:::: '''Preparation of 5000 U/mL stock solution''' (dissolved in H<sub>2</sub>O)
:::: Please check the concentration of the batch to calculate the volume needed to obtain a stock solution of 5000 U/mL using 2 mg of superoxide dismutase.
::::# Weigh 2 mg of superoxide dismutase.
::::# Calculate the volume needed to obtain a stock solution of 5000 U/mL (e.g. 1134 µL H<sub>2</sub>O for a batch with a concentration of 2835 U/mg).
::::# Divide into 30 µL portions into 0.2 mL Eppendorf tubes.
::::# Store frozen at -20 °C in the desiccator.
::::* Add SOD for conversion of O<sub>2</sub><sup>•-</sup> and [[Amplex_UltraRed|Measurement of H<sub>2</sub>O<sub>2</sub> with Amplex UltraRed]].
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