Stepanova 2020 Methods Cell Biol

Stepanova Anna, Galkin Alexander (2020) Measurement of mitochondrial H2O2 production under varying O2 tensions Methods Cell Biol [Epub ahead of print].

» DOI: https://doi.org/10.1016/bs.mcb.2019.12.008

Stepanova Anna, Galkin Alexander (2020) Methods Cell Biol

Abstract: Mitochondria-derived reactive oxygen species (ROS) play an important role in the development of several pathologies and are also involved in physiological signaling. Molecular oxygen is the direct substrate of Complex IV of the respiratory chain, and at the same time, its partial reduction in mitochondria results in the formation of ROS, mainly H₂O₂. The accurate knowledge of the dependence of H₂O₂ production on oxygen concentration is vital for the studies of tissue ischemia/reperfusion, where the relationship between oxygen availability, respiration, and ROS production is critical. In this chapter, we describe a straightforward and reliable protocol for the assessment of H₂O₂ release by mitochondria at varying oxygen concentrations. This method can be used for any ROS-generating system where the effect of oxygen level on H₂O₂ production needs to be assessed. Copyright © 2020 Elsevier B.V. All rights reserved.

• Bioblast editor: Komlodi T

Labels: MiParea: Respiration 

Organism: Mouse  Tissue;cell: Brain"Brain" is not in the list (Heart, Skeletal muscle, Nervous system, Liver, Kidney, Lung;gill, Islet cell;pancreas;thymus, Endothelial;epithelial;mesothelial cell, Blood cells, Fat, ...) of allowed values for the "Tissue and cell" property.  Preparation: Isolated mitochondria 

Coupling state: LEAK, OXPHOS  Pathway: N  HRR: Oxygraph-2k 

Labels, 2020-03