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Difference between revisions of "State 3"

From Bioblast
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|abbr=''P''
|abbr=''P''
|description=Whereas [[OXPHOS capacity]] is measured at saturating concentrations of ADP and Pi, '''State 3''' is defined as ADP stimulated respiration in the presence of high ADP and Pi concentrations [[Chance_1955_JBC-III|(Chance and Williams, 1955]]) which are not necessarily saturating.  For instance, non-saturating ADP concentrations are applied in State 3 in pulse titrations to determine the [[P/O ratio]] in State 3→4 (D→T) transitions, when saturating ADP concentrations would deplete the oxygen concentration in the closed oxygraph chamber before [[State 4]] is obtained ([[Gnaiger_2000_PNAS|Gnaiger et al 2000]]; [[Puchowicz_2004_Mitochondrion|(Puchowicz et al 2004]]).
|description=Whereas [[OXPHOS capacity]] is measured at saturating concentrations of ADP and Pi, '''State 3''' is defined as ADP stimulated respiration in the presence of high ADP and Pi concentrations [[Chance_1955_JBC-III|(Chance and Williams, 1955]]) which are not necessarily saturating.  For instance, non-saturating ADP concentrations are applied in State 3 in pulse titrations to determine the [[P/O ratio]] in State 3→4 (D→T) transitions, when saturating ADP concentrations would deplete the oxygen concentration in the closed oxygraph chamber before [[State 4]] is obtained ([[Gnaiger_2000_PNAS|Gnaiger et al 2000]]; [[Puchowicz_2004_Mitochondrion|(Puchowicz et al 2004]]).
|info=MiPNet12.15, Chance_1955_JBC-III
|info=MiPNet12.15
|type=Respiration
|type=Respiration
}}
}}

Revision as of 07:47, 16 September 2010


high-resolution terminology - matching measurements at high-resolution


State 3

Description

Whereas OXPHOS capacity is measured at saturating concentrations of ADP and Pi, State 3 is defined as ADP stimulated respiration in the presence of high ADP and Pi concentrations (Chance and Williams, 1955) which are not necessarily saturating. For instance, non-saturating ADP concentrations are applied in State 3 in pulse titrations to determine the P/O ratio in State 3→4 (D→T) transitions, when saturating ADP concentrations would deplete the oxygen concentration in the closed oxygraph chamber before State 4 is obtained (Gnaiger et al 2000; (Puchowicz et al 2004).

Abbreviation: P

Reference: MiPNet12.15


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Regulation: Respiration; OXPHOS; ETS Capacity"Respiration; OXPHOS; ETS Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property., ATP; ADP; AMP; PCr"ATP; ADP; AMP; PCr" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.