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Difference between revisions of "State 3"

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Whereas [[OXPHOS capacity]] is measured at saturating concentrations of ADP and Pi, State 3 is defined as ADP stimulated respiration in the presence of high ADP and Pi concentrations (Chance and Williams, 1955) which are not necessarily saturating.  For instance, non-saturating ADP concentrations are applied in State 3 in pulse titrations to determine the [[P/O ratio]] in State 3→4 (D→T) transitions, when saturating ADP concentrations would deplete the oxygen concentration in the closed oxygraph chamber before [[State 4]] is obtained (Gnaiger et al 2000; Puchowicz et al 2004).
{{MitoPedia
==References==
|abbr=''P''
*Chance B, Williams GR (1955) Respiratory enzymes in oxidative phosphorylation. III. The steady state. J. Biol. Chem. 217: 409-427.
|description=Whereas [[OXPHOS capacity]] is measured at saturating concentrations of ADP and Pi, '''State 3''' is defined as ADP stimulated respiration in the presence of high ADP and Pi concentrations [[(Chance_1955_JBC-III|Chance and Williams, 1955]]) which are not necessarily saturating.  For instance, non-saturating ADP concentrations are applied in State 3 in pulse titrations to determine the [[P/O ratio]] in State 3→4 (D→T) transitions, when saturating ADP concentrations would deplete the oxygen concentration in the closed oxygraph chamber before [[State 4]] is obtained ([[Gnaiger_2000_PNAS|Gnaiger et al 2000]]; [[Puchowicz_2004_Mitochondrion|(Puchowicz et al 2004]]).
*Gnaiger E, MΓ©ndez G, Hand SC (2000) High phosphorylation efficiency and depression of uncoupled respiration in mitochondria under hypoxia. [http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=27151&blobtype=pdf Proc. Natl. Acad. Sci. USA 97: 11080-11085].
|info=MiPNet12.15, Chance_1955_JBC-III
*Puchowicz MA, Varnes ME, Cohen BH, Friedman NR, Kerr DS, Hoppel CL (2004) Oxidative phosphorylation analysis: assessing the integrated functional activity of human skeletal muscle mitochondria – case studies. Mitochondrion 4: 377-385.
|type=Respiration
Β 
}}
{{MitoPedia}}
{{Labeling
|discipline=Mitochondrial Physiology
|topics=Respiration; OXPHOS; ETS Capacity, ATP; ADP; AMP; PCr
}}

Revision as of 07:47, 16 September 2010


high-resolution terminology - matching measurements at high-resolution


State 3

Description

Whereas OXPHOS capacity is measured at saturating concentrations of ADP and Pi, State 3 is defined as ADP stimulated respiration in the presence of high ADP and Pi concentrations Chance and Williams, 1955) which are not necessarily saturating. For instance, non-saturating ADP concentrations are applied in State 3 in pulse titrations to determine the P/O ratio in State 3→4 (D→T) transitions, when saturating ADP concentrations would deplete the oxygen concentration in the closed oxygraph chamber before State 4 is obtained (Gnaiger et al 2000; (Puchowicz et al 2004).

Abbreviation: P

Reference: MiPNet12.15, Chance_1955_JBC-III


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Regulation: Respiration; OXPHOS; ETS Capacity"Respiration; OXPHOS; ETS Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property., ATP; ADP; AMP; PCr"ATP; ADP; AMP; PCr" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.