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Property "Has-20abstract" (as page type) with input value "NADPH-20oxidase-20(Nox)-20is-20emerging-20as-20one-20of-20the-20major-20sources-20of-20cellular-20reactive-20oxygen-20species-20(ROS).-20While-20controlled-20ROS-20generation-20by-20Nox-20is-20involved-20in-20the-20redox-20regulation-20of-20physiological-20cellular-20processes,-20excessive-20ROS-20production-20leads-20to-20tissue-20damage-20-5B1-5D.-20Nox-20over-2Dreactivity-20has-20been-20shown-20to-20mediate-20the-20pathogenesis-20of-20tissue-20injury-20in-20neurodegenerative-20disorders-20-5B2-5D,-20ischemia-2Dreperfusion-20and-20cardiovascular-20disorders.-20Because-20of-20the-20short-2Dlived-20nature-20of-20ROS,-20it-20is-20challenging-20to-20assess-20and-20monitor-20ROS-20levels-20in-20biological-20specimens.-20Thus,-20the-20development-20of-20a-20method-20to-20measure-20NADPH-20oxidase-2Dderived-20ROS-20generation-20would-20be-20a-20valuable-20research-20tool-20to-20understand-20mechanisms-20relevant-20to-20neurodegeneration-20and-20tissue-20injury.-20Furthermore,-20this-20approach-20might-20be-20of-20relevance-20for-20screening-20of-20novel-20Nox-20inhibitors,-20which-20may-20selectively-20reduce-20disease-2Drelated-20Nox-2Dmediated-20ROS-20generation-20without-20modifying-20ROS-20physiological-20signaling-20function.-20-0A-0ABy-20using-20the-20Oroboros-20Oxygraph-2D2k,-20we-20applied-20two-20different-20protocols-20for-20measuring-20oxygen-20consumption-20in-20parallel-20with-20ROS-20levels-20in-20freshly-20isolated-20synaptosomes.-20In-20parallel-20with-20spin-20trapping-20EPR-20spectroscopy,-20we-20employed-20this-20protocol-20to-20delineate-20the-20contribution-20of-20NADPH-20oxidase-20to-20ROS-20production-20in-20young-20female-20and-20male-20C57BL6-20mice.-20The-20first-20protocol-20based-20on-20using-20a-20polarographic-20high-20resolution-20O2k-20sensor-20to-20measure-20oxygen-20consumption-20and-20a-20fluorescence-2Dbased-20module-20to-20monitor-20the-20rate-20of-20NADPH-2Dmediated-20hydrogen-20peroxide-20production.-20Consistent-20Nox-2Ddependent-20oxygen-20consumption-20was-20detected-20in-20synaptosomes-20following-20activation-20of-20Nox-20by-205-20mM-20NADPH-20(3-20doses).-20In-20parallel,-20we-20also-20employed-20a-20WPI-20-2Delectrochemical-20sensor-20to-20determine-20H2O2-20in-20the-20same-20sample.-20Although-20we-20didn't-20detect-20sex-2Ddependent-20discrepancy-20in-20the-20rate-20of-20hydrogen-20peroxide-20production-20by-20Nox-20in-20isolated-20synaptosomes,-20the-20HRP-2FAmplex-20Red-20system-20was-20associated-20with-20greater-20oxygen-20consumption-20and-20higher-20rates-20of-20hydrogen-20peroxide-20generation,-20suggesting-20that-20HRP-20may-20be-20inducing-20Nox-2Dlike-20activity.-20We-20verified-20the-20Nox-20activity-20using-20spin-20trapping-20EPR-20spectroscopy.-20Our-20study-20revealed-20that-20the-20Oroboros-20Oxygraph-2D2k-20can-20be-20successfully-20used-20for-20assessment-20of-20Nox-20activity-20through-20the-20parallel-20detection-20of-20oxygen-20consumption-20and-20the-20resulting-20hydrogen-20peroxide-20generation.-20However,-20we-20have-20also-20found-20that-20HRP-20exhibit-20NADPH-2Ddependent,-20oxygen-2Dconsuming,-20and-20H-3Csup-3E2-3C-2Fsup-3EO-3Csup-3E2-3C-2Fsup-3E-20-2Dproducing-20activity.-20Efforts-20are-20currently-20exerted-20to-20test-20other-20redox-2Dsensitive-20dyes-20for-20the-20detection-20of-20ROS-20in-20the-20absence-20of-20HRP." contains invalid characters or is incomplete and therefore can cause unexpected results during a query or annotation process.

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