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This page provides a simple browsing interface for finding entities described by a property and a named value. Other available search interfaces include the page property search, and the ask query builder.

Search by property

Property "Has-20abstract" (as page type) with input value "Amino-20acids-20participate-20in-20several-20critical-20processes-20in-20the-20biology-20of-20trypanosomatids,-20such-20as-20osmoregulation,-20cell-20differentiation,-20and-20host-20cell-20invasion.-20Some-20of-20them-20provide-20reducing-20power-20for-20mitochondrial-20ATP-20synthesis.-20It-20was-20previously-20shown-20that-20alanine,-20which-20is-20formed-20mainly-20by-20the-20amination-20of-20pyruvate,-20is-20a-20metabolic-20end-20product-20formed-20when-20parasites-20are-20replicating-20in-20a-20medium-20rich-20in-20glucose-20and-20amino-20acids.-20It-20was-20shown-20as-20well-20that-20this-20amino-20acid-20can-20also-20be-20used-20for-20the-20regulation-20of-20cell-20volume-20and-20resistance-20to-20osmotic-20stress.-20In-20this-20work,-20we-20demonstrate-20that,-20despite-20it-20being-20an-20end-20product-20of-20its-20metabolism,-20-27-27Trypanosoma-20cruzi-27-27-20can-20take-20up-20and-20metabolize-20l-2DAla-20through-20a-20low-2Dspecificity-20nonstereoselective-20active-20transport-20system.-20The-20uptake-20was-20dependent-20on-20the-20temperature-20in-20the-20range-20between-2010-20and-2040°C,-20which-20allowed-20us-20to-20calculate-20an-20activation-20energy-20of-2066.4-20kJ-2Fmol-20and-20estimate-20the-20number-20of-20transporters-20per-20cell-20at-20~436,000.-20We-20show-20as-20well-20that,-20once-20taken-20up-20by-20the-20cells,-20l-2DAla-20can-20be-20completely-20oxidized-20to-20CO-3Csub-3E2-3C-2Fsub-3E,-20supplying-20electrons-20to-20the-20electron-20transport-20chain,-20maintaining-20the-20electrochemical-20proton-20gradient-20across-20the-20mitochondrial-20inner-20membrane,-20and-20supporting-20ATP-20synthesis-20in-20-27-27T.-20cruzi-27-27-20epimastigotes.-20Our-20data-20demonstrate-20a-20dual-20role-20for-20Ala-20in-20the-20parasite's-20bioenergetics,-20by-20being-20a-20secreted-20end-20product-20of-20glucose-20catabolism-20and-20taken-20up-20as-20nutrient-20for-20oxidative-20mitochondrial-20metabolism.-0A-0AIt-20is-20well-20known-20that-20trypanosomatids-20such-20as-20the-20etiological-20agent-20of-20Chagas'-20disease,-20-27-27Trypanosoma-20cruzi-27-27,-20produce-20alanine-20as-20a-20main-20end-20product-20of-20their-20energy-20metabolism-20when-20they-20grow-20in-20a-20medium-20containing-20glucose-20and-20amino-20acids.-20In-20this-20work,-20we-20investigated-20if-20under-20starvation-20conditions-20(which-20happen-20during-20the-20parasite-20life-20cycle)-20the-20secreted-20alanine-20could-20be-20recovered-20from-20the-20extracellular-20medium-20and-20used-20as-20an-20energy-20source.-20Herein-20we-20show-20that-20indeed,-20in-20parasites-20submitted-20to-20metabolic-20stress,-20this-20metabolite-20can-20be-20taken-20up-20and-20used-20as-20an-20energy-20source-20for-20ATP-20synthesis,-20allowing-20the-20parasite-20to-20extend-20its-20survival-20under-20starvation-20conditions.-20The-20obtained-20results-20point-20to-20a-20dual-20role-20for-20Ala-20in-20the-20parasite's-20bioenergetics,-20by-20being-20a-20secreted-20end-20product-20of-20glucose-20catabolism-20and-20taken-20up-20as-20nutrient-20for-20oxidative-20mitochondrial-20metabolism." contains invalid characters or is incomplete and therefore can cause unexpected results during a query or annotation process.

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