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• Affourtit 1999 J Biol Chem  + (We have investigated the extent to which f … We have investigated the extent to which functional expression of the plant alternative oxidase (from ''Sauromatum guttatum'') in ''Schizosaccharomyces pombe'' affects yeast growth. When cells are cultured on glycerol, the maximum specific growth rate is decreased from 0.13 to 0.11 h^-1 while growth yield is lowered by 20% (from 1. 14 x 10⁸ to 9.12 x 10⁷ cells ml^-1). Kinetic studies suggest that the effect on growth is mitochondrial in origin. In isolated mitochondria we found that the alternative oxidase actively competes with the cytochrome pathway for reducing equivalents and contributes up to 24% to the overall respiratory activity. Metabolic control analysis reveals that the alternative oxidase exerts a considerable degree of control (22%) on total electron flux. Furthermore, the negative control exerted by the alternative oxidase on the flux ratio of electrons through the cytochrome and alternative pathways is comparable with the positive control exerted on this flux-ratio by the cytochrome pathway. To our knowledge, this is the first paper to report a phenotypic effect because of plant alternative oxidase expression. We suggest that the effect on growth is the result of high engagement of the non-protonmotive alternative oxidase in yeast respiration that, consequently, lowers the efficiency of energy conservation and hence growth.iration that, consequently, lowers the efficiency of energy conservation and hence growth.)

• Affourtit 1999 J Biol Chem  +