]>
2020-09-19T17:11:39+02:00
Cell ergometry
0
en
LEAK
OXPHOS
ET
Biochemical '''cell ergometry''' aims at measurement of ''J''<sub>O<sub>2</sub>max</sub> (compare ''V''<sub>O<sub>2</sub>max</sub> or ''V''<sub>O<sub>2</sub>peak</sub> in exercise ergometry of humans and animals) of cell respiration linked to phosphorylation of ADP to ATP. The corresponding [[OXPHOS capacity]] is based on saturating concentrations of ADP, [ADP]*, and inorganic phosphate, [Pi]*, available to the mitochondria. This is metabolically opposite to uncoupling respiration, which yields [[ET capacity]]. The OXPHOS state can be established experimentally by selective [[permeabilized cells |permeabilization of cell membranes]] with maintenance of intact mitochondria, titrations of ADP and P<sub>i</sub> to evaluate kinetically saturating conditions, and establishing fuel substrate combinations which reconstitute physiological [[TCA cycle]] function. Uncoupler titrations are applied to determine the apparent ET-pathway excess over OXPHOS capacity and to calculate [[OXPHOS-coupling efficiency |OXPHOS-]] and [[ET-coupling efficiency ]], ''j<sub>≈P</sub>'' and ''j<sub>≈E</sub>''. These normalized flux ratios are the basis to calculate the ergometric or [[ergodynamic efficiency]], ''ε'' = ''j'' · ''f'', where ''f'' is the normalized force ratio.
» [[Cell_ergometry#Cell_ergometry_and_OXPHOS|'''MiPNet article''']]
Spiroergometry on the organismic level is compared to cell ergometry as OXPHOS analysis on the cellular level.
Gnaiger E (2015) Cell ergometry and OXPHOS. Mitochondr Physiol Network 2015-01-18.
[[Gnaiger 2020 MitoPathways]], [[Oxygen flux]]
Theory
MiP concept
Ergodynamics
Respirometry
N
S
NS
ROX
Coupling efficiency;uncoupling
2015
Cell ergometry
2020-06-08T13:13:06Z
2459009.0507639
Cell ergometry