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Revision as of 13:44, 23 April 2012 by Eigentler Andrea (talk | contribs) (Created page with "{{MitoPedia |abbr=Na-PB |description= Sodium phosphate buffer, '''Na-PB''', for HRR with freeze-dried baker´s yeast |type=Chemicals }} {{MitoPedia methods |mitopedia method...")
Description
Sodium phosphate buffer, Na-PB, for HRR with freeze-dried baker´s yeast
Abbreviation: Na-PB
MitoPedia methods:
Respirometry
MitoPedia topics:
Media for respirometry
Application in HRR
- experimental temperature = 28°C
- Oxygen solubility factor of Na-PB = 0.95
== Na-PB (50 mM, pH 7.1) Dissolve 3.9 g H2NaO4P x 2 H2O in 500 ml a. dest and adjust pH with 10 M NaOH to 7.1.
- pH of Na-PB = 7.1