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Sodium phosphate buffer

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Revision as of 13:44, 23 April 2012 by Eigentler Andrea (talk | contribs) (Created page with "{{MitoPedia |abbr=Na-PB |description= Sodium phosphate buffer, '''Na-PB''', for HRR with freeze-dried baker´s yeast |type=Chemicals }} {{MitoPedia methods |mitopedia method...")
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Sodium phosphate buffer

Description

Sodium phosphate buffer, Na-PB, for HRR with freeze-dried baker´s yeast

Abbreviation: Na-PB


MitoPedia methods: Respirometry 


MitoPedia topics: Media for respirometry 


Application in HRR

  • experimental temperature = 28°C
  • Oxygen solubility factor of Na-PB = 0.95

== Na-PB (50 mM, pH 7.1) Dissolve 3.9 g H2NaO4P x 2 H2O in 500 ml a. dest and adjust pH with 10 M NaOH to 7.1.

  • pH of Na-PB = 7.1