Difference between revisions of "Skemiene 2020 J Bioenerg Biomembr"
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|keywords=Anthocyanins, Brain ischemia, Cell death, Mitochondria, Neuroprotection, Respiration | |keywords=Anthocyanins, Brain ischemia, Cell death, Mitochondria, Neuroprotection, Respiration | ||
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|mipnetlab=LT Kaunas Borutaite V | |||
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|area=Respiration | |area=Respiration, Pharmacology;toxicology | ||
|injuries=Ischemia-reperfusion | |||
|organism=Rat | |||
|tissues=Nervous system | |||
|preparations=Isolated mitochondria | |||
|couplingstates=LEAK, OXPHOS | |||
|pathways=N | |||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|additional=Labels, 2020-03, | |additional=Labels, 2020-03, | ||
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Revision as of 16:01, 9 March 2020
| Skemiene K, Pampuscenko K, Rekuviene E, Borutaite V (2020) Protective effects of anthocyanins against brain ischemic damage. J Bioenerg Biomembr [Epub ahead of print]. |
Skemiene K, Pampuscenko K, Rekuviene E, Borutaite V (2020) J Bioenerg Biomembr
Abstract: Anthocyanins are considered as bioactive components of plant-based diets that provide protection against ischemic cardiovascular pathologies by mechanisms dependent on their antioxidant and reductive capacities. However, it is not clear whether similar anthocyanin-mediated mechanisms can provide protection against ischemia-induced brain mitochondrial injury and cell death. In this study, we compared effects of three cyanidin-3-glycosides - glucoside (Cy3G), galactoside (Cy3Gal) and rutinoside (Cy3R), with pelargonxidin-3-glucoside (Pg3G) and found that at 10-20 μM concentrations they have no direct effect on respiratory functions of mitochondria isolated from normal or ischemia-damaged rat brain slices. However, intravenous injection of Cy3Gal and Cy3G (0,025 mg/kg or 0,05 mg/kg what matches 10 μM or 20 μM respectively) but not Cy3R in rats protected against ischemia-induced caspase activation and necrotic cell death, and reduced infarct size in cerebral cortex and cerebellum. These effects correlated with cytochrome c reducing capacity of cyanidin-3-glycosides. In contrast, intravenous injection of 0,025 mg/kg Pg3G which has the lowest cytochrome c reducing capacity among investigated anthocyanins, had no effect on ischemia-induced caspase activation and necrosis but reduced brain infarct size whereas intravenous injection of 0,05 mg/kg of Pg3G slightly promoted necrosis in the brain. Our data suggest that reductive rather than antioxidant capacities of anthocyanins may be important components in providing protection against ischemic brain damage. • Keywords: Anthocyanins, Brain ischemia, Cell death, Mitochondria, Neuroprotection, Respiration • Bioblast editor: Plangger M • O2k-Network Lab: LT Kaunas Borutaite V
Labels: MiParea: Respiration, Pharmacology;toxicology
Stress:Ischemia-reperfusion Organism: Rat Tissue;cell: Nervous system Preparation: Isolated mitochondria
Coupling state: LEAK, OXPHOS
Pathway: N
HRR: Oxygraph-2k
Labels, 2020-03
