Difference between revisions of "Skalska 2009 Int J Mol Sci"
| Line 4: | Line 4: | ||
|year=2009 | |year=2009 | ||
|journal=International Journal of Molecular Sciences | |journal=International Journal of Molecular Sciences | ||
|abstract=Abstract: The mitochondrial response to changes of cytosolic calcium concentration has a | |||
strong impact on neuronal cell metabolism and viability. We observed that Ca2+ additions | |||
to isolated rat brain mitochondria induced in potassium ion containing media a | |||
mitochondrial membrane potential depolarization and an accompanying increase of | |||
mitochondrial respiration. These Ca2+ effects can be blocked by iberiotoxin and | |||
charybdotoxin, well known inhibitors of large conductance potassium channel (BKCa | |||
channel). Furthermore, NS1619 – a BKCa channel opener – induced potassium ion–specific | |||
effects on brain mitochondria similar to those induced by Ca2+. These findings suggest the | |||
OPEN ACCESS | |||
Int. J. Mol. Sci. 2009, 10 | |||
1105 | |||
presence of a calcium-activated, large conductance potassium channel (sensitive to | |||
charybdotoxin and NS1619), which was confirmed by reconstitution of the mitochondrial | |||
inner membrane into planar lipid bilayers. The conductance of the reconstituted channel | |||
was 265 pS under gradient (50/450 mM KCl) conditions. Its reversal potential was equal to | |||
50 mV, which proved that the examined channel was cation-selective. We also observed | |||
immunoreactivity of anti-4 subunit (of the BKCa channel) antibodies with ~26 kDa | |||
proteins of rat brain mitochondria. Immunohistochemical analysis confirmed the | |||
predominant occurrence of 4 subunit in neuronal mitochondria. We hypothesize that the | |||
mitochondrial BKCa channel represents a calcium sensor, which can contribute to neuronal | |||
signal transduction and survival. | |||
|keywords=Mitochondria, Brain, Channel openers, Potassium channel, Iberiotoxin, NS1619. | |||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/19399240 PMID: 19399240] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/19399240 PMID: 19399240] | ||
}} | }} | ||
Revision as of 15:33, 21 September 2010
| Skalska J, Bednarczyk P, Piwonska M, Kulawiak B, Wilczynski G, Dolowy K, Kudin AP, Kunz WS, Szewczyk A (2009) Calcium Ions Regulate K Uptake into Brain Mitochondria: The Evidence for a Novel Potassium Channel. Int. J. Mol. Sci. 10: 1104-1120. |
Skalska J, Bednarczyk P, Piwonska M, Kulawiak B, Wilczynski G, Dolowy K, Kudin AP, Kunz WS, Szewczyk A (2009) International Journal of Molecular Sciences
Abstract: Abstract: The mitochondrial response to changes of cytosolic calcium concentration has a strong impact on neuronal cell metabolism and viability. We observed that Ca2+ additions to isolated rat brain mitochondria induced in potassium ion containing media a mitochondrial membrane potential depolarization and an accompanying increase of mitochondrial respiration. These Ca2+ effects can be blocked by iberiotoxin and charybdotoxin, well known inhibitors of large conductance potassium channel (BKCa channel). Furthermore, NS1619 – a BKCa channel opener – induced potassium ion–specific effects on brain mitochondria similar to those induced by Ca2+. These findings suggest the OPEN ACCESS Int. J. Mol. Sci. 2009, 10 1105 presence of a calcium-activated, large conductance potassium channel (sensitive to charybdotoxin and NS1619), which was confirmed by reconstitution of the mitochondrial inner membrane into planar lipid bilayers. The conductance of the reconstituted channel was 265 pS under gradient (50/450 mM KCl) conditions. Its reversal potential was equal to 50 mV, which proved that the examined channel was cation-selective. We also observed immunoreactivity of anti-4 subunit (of the BKCa channel) antibodies with ~26 kDa proteins of rat brain mitochondria. Immunohistochemical analysis confirmed the predominant occurrence of 4 subunit in neuronal mitochondria. We hypothesize that the mitochondrial BKCa channel represents a calcium sensor, which can contribute to neuronal signal transduction and survival. • Keywords: Mitochondria, Brain, Channel openers, Potassium channel, Iberiotoxin, NS1619.
Labels:
Regulation: Respiration; OXPHOS; ETS Capacity"Respiration; OXPHOS; ETS Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.
HRR: Oxygraph-2k
