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Difference between revisions of "Seppet 2005 Mol Cell Biochem"

From Bioblast
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{{Labeling
{{Labeling
|instruments=Oxygraph-2k
|instruments=Oxygraph-2k
|injuries=Mitochondrial Disease; Degenerative Disease and Defect
|organism=Human
|tissues=Cardiac Muscle
|preparations=Permeabilized Cell or Tissue; Homogenate
|preparations=Permeabilized Cell or Tissue; Homogenate
|topics=Respiration; OXPHOS; ETS Capacity
|topics=Respiration; OXPHOS; ETS Capacity
|discipline=Biomedicine
|discipline=Biomedicine
}}
}}

Revision as of 14:05, 17 November 2011

Publications in the MiPMap
Seppet E, Eimre M, Peet N, Paju K, Orlova E, Ress M, Kovask S, Piirsoo A, Saks VA, Gellerich FN, Zierz S, Seppet EK (2005) Compartmentation of energy metabolism in atrial myocardium of patients undergoing cardiac surgery. Mol. Cell. Biochem. 270: 49-61.

Β» PMID: 15792353

Seppet E, Eimre M, Peet N, Paju K, Orlova E, Ress M, Kovask S, Piirsoo A, Saks VA, Gellerich FN, Zierz S, Seppet EK (2005) Mol. Cell. Biochem.

Abstract: The parameters of oxidative phosphorylation and its interaction with creatine kinase (CK)- and adenylate kinase (AK)-phosphotransfer networks in situ were studied in skinned atrial fibers from 59 patients undergoing coronary artery bypass surgery, valve replacement/correction and atrial septal defect correction. In atria, the mitochondrial CK and AK are effectively coupled to oxidative phosphorylation, the MM-CK is coupled to ATPases and there exists a direct transfer of adenine nucleotides between mitochondria and ATPases. Elimination of cytoplasmic ADP with exogenous pyruvate kinase was not associated with a blockade of the stimulatory effects of creatine and AMP on respiration, neither could it abolish the coupling of MM-CK to ATPases and direct transfer of adenine nucleotides. Thus, atrial energy metabolism is compartmentalized so that mitochondria form functional complexes with adjacent ATPases. These complexes isolate a part of cellular adenine nucleotides from their cytoplasmic pool for participating in energy transfer via CK- and AK-networks, and/or direct exchange. Compared to atria in sinus rhythm, the fibrillating atria were larger and exhibited increased succinate-dependent respiration relative to glutamate-dependent respiration and augmented proton leak. Thus, alterations in mitochondrial oxidative phosphorylation may contribute to pathogenesis of atrial fibrillation. β€’ Keywords: Skinned fibers, Human myocardium, Mitochondria, Oxidative phosphorylation, Phosphotransfer networks

β€’ O2k-Network Lab: EE_Tartu_Seppet EK


Labels:


Organism: Human  Tissue;cell: Cardiac Muscle"Cardiac Muscle" is not in the list (Heart, Skeletal muscle, Nervous system, Liver, Kidney, Lung;gill, Islet cell;pancreas;thymus, Endothelial;epithelial;mesothelial cell, Blood cells, Fat, ...) of allowed values for the "Tissue and cell" property.  Preparation: Permeabilized Cell or Tissue; Homogenate"Permeabilized Cell or Tissue; Homogenate" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property. 

Regulation: Respiration; OXPHOS; ETS Capacity"Respiration; OXPHOS; ETS Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property. 


HRR: Oxygraph-2k