Difference between revisions of "Schulte 2013 Abstract MiP2013"
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{{Abstract | {{Abstract | ||
|title=Chung DJ, Schulte PM, Richards JG (2013) The effect of low-temperature acclimation on mitochondrial function in the common killifish (''Fundulus heteroclitus''), a top-down elasticity analysis. Mitochondr Physiol Network 18.08. | |title=Chung DJ, Schulte PM, Richards JG (2013) The effect of low-temperature acclimation on mitochondrial function in the common killifish (''Fundulus heteroclitus''), a top-down elasticity analysis. Mitochondr Physiol Network 18.08. | ||
|info=[[File:SchulteP.jpg|120px|right|Patricia Schulte]][ | |info=[[File:SchulteP.jpg|120px|right|Patricia Schulte]][[MiP2013]], [[Laner 2013 Mitochondr Physiol Network MiP2013|Book of Abstracts Open Access]] | ||
|authors=Chung DJ, Schulte PM, Richards JG | |authors=Chung DJ, Schulte PM, Richards JG | ||
|year=2013 | |year=2013 | ||
|event= | |event=MiPNet18.08_MiP2013 | ||
|abstract=Based on its presumed role in altering whole-animal metabolic rate, the mitochondrion has become the focus of hypotheses that address the process of thermal adaptation. It has been proposed that temperature-induced limitations on mitochondrial function (due to passive thermal effects on biochemical activity or an imbalance between O2 supply and demand at low temperatures) affect whole organism performance and, as a result, cold-adapted or –acclimated species compensate with increased mitochondrial density and/or activity [1]. To address this hypothesis we use Fundulus heteroclitus, a teleost species with genetically distinct, locally adapted subpopulations (Northern, Southern, and Hybrid) which reside over a large thermal gradient. During acute high temperature shifts (37 °C), liver mitochondria isolated from 5 °C acclimated Northern Fundulus heteroclitus lose the capacity to perform oxidative phosphorylation. This phenomenon is not observed with fish acclimated to 15 and 25 °C, which is indicative of a cost of acclimation to low temperatures [2]. | |abstract=Based on its presumed role in altering whole-animal metabolic rate, the mitochondrion has become the focus of hypotheses that address the process of thermal adaptation. It has been proposed that temperature-induced limitations on mitochondrial function (due to passive thermal effects on biochemical activity or an imbalance between O2 supply and demand at low temperatures) affect whole organism performance and, as a result, cold-adapted or –acclimated species compensate with increased mitochondrial density and/or activity [1]. To address this hypothesis we use Fundulus heteroclitus, a teleost species with genetically distinct, locally adapted subpopulations (Northern, Southern, and Hybrid) which reside over a large thermal gradient. During acute high temperature shifts (37 °C), liver mitochondria isolated from 5 °C acclimated Northern Fundulus heteroclitus lose the capacity to perform oxidative phosphorylation. This phenomenon is not observed with fish acclimated to 15 and 25 °C, which is indicative of a cost of acclimation to low temperatures [2]. | ||
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{{Labeling | {{Labeling | ||
|area=Respiration, Comparative MiP;environmental MiP | |area=Respiration, Comparative MiP;environmental MiP | ||
| | |organism=Fishes | ||
|tissues=Liver | |tissues=Liver | ||
|preparations=Isolated | |preparations=Isolated mitochondria | ||
|injuries=RONS | |injuries=Oxidative stress;RONS | ||
|topics=mt-Membrane potential, Temperature | |topics=mt-Membrane potential, Temperature | ||
|couplingstates=LEAK, OXPHOS | |couplingstates=LEAK, OXPHOS |
Latest revision as of 11:10, 28 April 2017
Chung DJ, Schulte PM, Richards JG (2013) The effect of low-temperature acclimation on mitochondrial function in the common killifish (Fundulus heteroclitus), a top-down elasticity analysis. Mitochondr Physiol Network 18.08. |
Link:
MiP2013, Book of Abstracts Open Access
Chung DJ, Schulte PM, Richards JG (2013)
Event: MiPNet18.08_MiP2013
Based on its presumed role in altering whole-animal metabolic rate, the mitochondrion has become the focus of hypotheses that address the process of thermal adaptation. It has been proposed that temperature-induced limitations on mitochondrial function (due to passive thermal effects on biochemical activity or an imbalance between O2 supply and demand at low temperatures) affect whole organism performance and, as a result, cold-adapted or –acclimated species compensate with increased mitochondrial density and/or activity [1]. To address this hypothesis we use Fundulus heteroclitus, a teleost species with genetically distinct, locally adapted subpopulations (Northern, Southern, and Hybrid) which reside over a large thermal gradient. During acute high temperature shifts (37 °C), liver mitochondria isolated from 5 °C acclimated Northern Fundulus heteroclitus lose the capacity to perform oxidative phosphorylation. This phenomenon is not observed with fish acclimated to 15 and 25 °C, which is indicative of a cost of acclimation to low temperatures [2].
To investigate the functional differences in mitochondrial properties as a result of low temperature acclimation we have acclimated Northern and Southern Fundulus heteroclitus to 5, 15 and 33 °C. We compare the kinetics of liver mitochondrial ADP-phosphorylation, proton conductance, and substrate oxidation during acute shifts to 5, 15, and 33 °C. In addition, we compare the rates of basal and maximum reactive oxygen species (ROS) production to assess its contribution as a result of proton conductance.
Our current results indicate that during acute shifts to high temperature, cold-acclimated Northern killifish exhibit equivalent levels of LEAK respiration (i.e., proton leak) as room- and warm-temperature acclimated killifish while maintaining a lower membrane potential. This equivalent level of proton leak is reflected in no difference in ROS production when compared to the 15 °C acclimation. In addition, warm-acclimation appears to result in increased basal ROS production, while lowering maximal ROS. These results indicate that there are changes in mitochondrial function associated with low-temperature acclimation.
• O2k-Network Lab: CA Vancouver Richards JG
Labels: MiParea: Respiration, Comparative MiP;environmental MiP
Stress:Oxidative stress;RONS Organism: Fishes Tissue;cell: Liver Preparation: Isolated mitochondria
Regulation: mt-Membrane potential, Temperature Coupling state: LEAK, OXPHOS
HRR: Oxygraph-2k
MiP2013, S04
Affiliations and author contributions
Department of Zoology, University of British Columbia; Vancouver, Canada. - Email: [email protected]
References
- Pörtner HO (2010) Oxygen- and capacity-limitation of thermal tolerance: a matrix for integrating climate-related stressor effects in marine ecosystems. J Exp Biol 213: 881-893.
- Fangue NA, Richards JG, Schulte PM (2009) Do mitochondrial properties explain intraspecific variation in thermal tolerance? J Exp Biol 212: 514–522.