Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Difference between revisions of "Sant'Anna-Silva 2018 Front Oncol"

From Bioblast
ย 
Line 14: Line 14:
|diseases=Cancer
|diseases=Cancer
|organism=Human
|organism=Human
|tissues=Other cell lines
|tissues=Other cell lines, Fibroblast
|preparations=Intact cells
|preparations=Intact cells
|topics=Inhibitor
|topics=Inhibitor

Latest revision as of 12:26, 21 August 2018

Publications in the MiPMap
Sant'Anna-Silva ACB, Santos GC, Campos SPC, Oliveira Gomes AM, Pรฉrez-Valencia JA, Rumjanek FD (2018) Metabolic profile of oral squamous carcinoma cell lines relies on a higher demand of lipid metabolism in metastatic cells. Front Oncol 8:13.

ยป PMID: 29456966 Open Access

Sant'Anna-Silva ACB, Santos GC, Campos SPC, Oliveira Gomes AM, Perez Valencia JA, Rumjanek FD (2018) Front Oncol

Abstract: Tumor cells are subjected to a broad range of selective pressures. As a result of the imposed stress, subpopulations of surviving cells exhibit individual biochemical phenotypes that reflect metabolic reprograming. The present work aimed at investigating metabolic parameters of cells displaying increasing degrees of metastatic potential. The metabolites present in cell extracts fraction of tongue fibroblasts and of cell lines derived from human tongue squamous cell carcinoma lineages displaying increasing metastatic potential (SCC9 ZsG, LN1 and LN2) were analyzed by 1H NMR (nuclear magnetic resonance) spectroscopy. Living, intact cells were also examined by the non-invasive method of fluorescence lifetime imaging microscopy (FLIM) based on the auto fluorescence of endogenous NADH. The cell lines reproducibly exhibited distinct metabolic profiles confirmed by Partial Least-Square Discriminant Analysis (PLS-DA) of the spectra. Measurement of endogenous free and bound NAD(P)H relative concentrations in the intact cell lines showed that ZsG and LN1 cells displayed high heterogeneity in the energy metabolism, indicating that the cells would oscillate between glycolysis and oxidative metabolism depending on the microenvironment's composition. However, LN2 cells appeared to have more contributions to the oxidative status, displaying a lower NAD(P)H free/bound ratio. Functional experiments of energy metabolism, mitochondrial physiology, and proliferation assays revealed that all lineages exhibited similar energy features, although resorting to different bioenergetics strategies to face metabolic demands. These differentiated functions may also promote metastasis. We propose that lipid metabolism is related to the increased invasiveness as a result of the accumulation of malonate, methyl malonic acid, n-acetyl and unsaturated fatty acids (CH2)n in parallel with the metastatic potential progression, thus suggesting that the NAD(P)H reflected the lipid catabolic/anabolic pathways. โ€ข Keywords: Cancer progression, Metabolic reprogramming, Metabolomics, Metastasis, Nuclear magnetic resonance, Oral squamous cell carcinoma โ€ข Bioblast editor: Kandolf G, Sant'Anna-Silva ACB โ€ข O2k-Network Lab: BR Rio de Janeiro Rumjanek FD


Labels: MiParea: Respiration  Pathology: Cancer 

Organism: Human  Tissue;cell: Other cell lines, Fibroblast  Preparation: Intact cells 

Regulation: Inhibitor  Coupling state: LEAK, ROUTINE, ET  Pathway: ROX  HRR: Oxygraph-2k 

Labels, 2018-02