Difference between revisions of "Safranin"
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::::# Divide into 0.5 mL portions (use dark vials); | ::::# Divide into 0.5 mL portions (use dark vials); | ||
::::# Store at 4 °C. | ::::# Store at 4 °C. | ||
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:::: At a concentration of 2 µM, safranin can be applied for simultaneous monitoring of oxygen consumption and mtMP of mt-preparations in a CII-linked substrate state.<ref> Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542:163-81. [[Krumschnabel 2014 Methods Enzymol |»Bioblast link«]]</ref> | :::: At a concentration of 2 µM, safranin can be applied for simultaneous monitoring of oxygen consumption and mtMP of mt-preparations in a CII-linked substrate state.<ref> Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542:163-81. [[Krumschnabel 2014 Methods Enzymol |»Bioblast link«]]</ref> | ||
Revision as of 11:51, 14 August 2018
- high-resolution terminology - matching measurements at high-resolution
Safranin
Description
Safranin is one of the most established dyes for measuring mitochondrial membrane potential by fluorometry. It is an extrinsic fluorophore with an excitation wavelength of 495 nm and emission wavelength of 587 nm. Safranin is a potent inhibitor of Complex I-linked respiration and of the phosphorylation system.
Abbreviation: Saf
Reference: Kauppinen 1984 Am J Physiol, Krumschnabel 2014 Methods Enzymol, MiPNet20.13 Safranin mt-membranepotential
MitoPedia methods:
Fluorometry
Synonyms
- Safranin O, Safranin Y, Saranin T, Gossypimine, Cotton Red, Basic Red2
Signal and output
- Signal: The O2k-Fluo LED2-Module is operated through the amperometric (Amp)-Channel of the O2k, with electric current (ampere [A]) as the primary signal.
- Output: The focus of the output with Safranin is on Type C: Force, mt-membrane potential.
Application in HRFR
- Safranin O (C20H19ClN4)
- Sigma S2255, 25 or 100 mg, store at -20°C; FW = 350.84
Caution: Light sensitive (store solution in a dark vial)!
- Preparation of 1 mM solution - use for experiment:
- Weight 3.508 mg of Safranin and dissolve in distilled water to reach 10 mL final volume;
- Divide into 0.5 mL portions (use dark vials);
- Store at 4 °C.
- Preparation of 1 mM solution - use for experiment:
-
- At a concentration of 2 µM, safranin can be applied for simultaneous monitoring of oxygen consumption and mtMP of mt-preparations in a CII-linked substrate state.[1]
O2k-Protocol: O2k-Fluorometry: HRR and simultaneous determination of mt-membrane potential with safranin. »MiPNet20.13«
