SUIT protocol library
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Substrate-uncoupler-inhibitor titration (SUIT) protocols
Classes of SUIT protocols with coupling and substrate control
Definition of states
Define substrates
- CI: PM, GM, PGM, M, ..
- CII: S(Rot), S, SM, ..
- CI&II: PS, GS, PMS, GMS, PGMS, ..
- CIV: AsTM
- FAO: Oct, Pal, ...
- CGPDH:
- c
Define coupling states
- L, P, E
Define residual oxygen consumption
- ROX: Rot&Ama, Rot&Mna&Ama, Cn, Az, ..
Substrate control states
Single substrate control states
- CI
- CII
- CI&CII
- CIV
- FAO
- CGPDH
Multiple substrate control states
A. CI-CII-CIV
- CI + CII
- CI + CII + CIV
- CI + CI&CII + CII
- CI + CI&CII + CII + CIV
B. FAO-CI-CII-CIV
- FAO + CI + CII
- FAO + CI + CII + CIV
- FAO + CI + CI&CII + CII
- FAO + CI + CI&CII + CII + CIV
C. CGPDH-CI-CII-CIV
- CGPDH + CI
- CGPDH + CI + CII
- CGPDH + CI + CII + CIV
- CGPDH + CI + CI&CII + CII
- CGPDH + CI + CI&CII + CII + CIV
- CI + CGPDH + ...
- CI + CI&CII + CGPDH + ...
- CI&CII + CGPDH + ...
D. FAO-CGPDH-CI-CII-CIV
- FAO + CGPDH + CI + CII
- FAO + CGPDH + CI + CII + CIV
- FAO + CGPDH + CI + CI&CII + CII
- FAO + CGPDH + CI + CI&CII + CII + CIV
- ...
Control patterns
Linear
- Linear coupling control: at a single substrate state (classical State 3 -> State 4, or P -> LT).
- Linear substrate control: at a single coupling state (unusual).
- Monodirectional linear coupling control: LN -> PD -> EF
- Bidirectional linear coupling control: LN -> PD -> LOmy -> EF
Orthogonal
- Orthogonal coupling -> substrate control (mono- or bidirectional)
- Orthogonal substrate -> coupling control (mono- or bidirectional)
Diametral
- Diametral coupling -> substrate ->> control (mono- or bidirectional)
- Diametral substrate -> coupling ->> control (mono- or bidirectional)
