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Difference between revisions of "SUIT protocol library"

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'''''This page is in preparation''''' (intern wiki: Library of protocols)
'''''This page is in preparation''''' (intern wiki: SUIT protocol library intern)


= Classes of protocols with coupling and substrate control =
<big><big>'''[[Substrate-uncoupler-inhibitor titration]] (SUIT) protocols


== Linear ==
Classes of SUIT protocols with coupling and substrate control'''</big></big>


# '''Linear coupling control''': at a single substrate state (classical State 3 -> State 4).
== Definition of states ==
 
=== Define substrates ===
* CI: PM, GM, PGM, M, ..
* CII: S(Rot), S, SM, ..
* CI&II: PS, GS, PMS, GMS, PGMS, ..
* CIV: AsTM
* FAO: Oct, Pal, ...
* CGPDH:
* c
 
=== Define coupling states ===
* ''L'', ''P'', ''E''
 
=== Define residual oxygen consumption ===
* ROX: Rot&Ama, Rot&Mna&Ama, Cn, Az, ..
 
 
== Substrate control states ==
 
=== Single substrate control states ===
 
# CI
# CII
# CI<small>&</small>CII
# CIV
# FAO
# CGPDH
 
=== Multiple substrate control states ===
 
'''A. CI-CII-CIV'''
# CI + CII
# CI + CII + CIV
# CI + CI<small>&</small>CII + CII
# CI + CI<small>&</small>CII + CII + CIV
 
'''B. FAO-CI-CII-CIV'''
# FAO + CI + CII
# FAO + CI + CII + CIV
# FAO + CI + CI<small>&</small>CII + CII
# FAO + CI + CI<small>&</small>CII + CII + CIV
 
'''C. CGPDH-CI-CII-CIV'''
# CGPDH + CI
# CGPDH + CI + CII
# CGPDH + CI + CII + CIV
# CGPDH + CI + CI<small>&</small>CII + CII
# CGPDH + CI + CI<small>&</small>CII + CII + CIV
# CI + CGPDH + ...
# CI + CI<small>&</small>CII + CGPDH + ...
# CI<small>&</small>CII + CGPDH + ...
 
'''D. FAO-CGPDH-CI-CII-CIV'''
# FAO + CGPDH + CI + CII
# FAO + CGPDH + CI + CII + CIV
# FAO + CGPDH + CI + CI<small>&</small>CII + CII
# FAO + CGPDH + CI + CI<small>&</small>CII + CII + CIV
# ...
 
 
== Control patterns ==
=== Linear ===
 
# '''Linear coupling control''': at a single substrate state (classical State 3 -> State 4, or ''P'' -> ''L''<sub>T</sub>).
# '''Linear substrate control''': at a single coupling state (unusual).
# '''Linear substrate control''': at a single coupling state (unusual).
# '''Monodirectional linear coupling control''': ''L''<sub>N</sub> -> ''P''<sub>D</sub> -> ''E''<sub>F</sub>
# '''Monodirectional linear coupling control''': ''L''<sub>N</sub> -> ''P''<sub>D</sub> -> ''E''<sub>F</sub>
# '''Bidirectional linear coupling control''': ''L''<sub>N</sub> -> ''P''<sub>D</sub> -> ''L''<sub>Omy</sub> -> ''E''<sub>F</sub>
# '''Bidirectional linear coupling control''': ''L''<sub>N</sub> -> ''P''<sub>D</sub> -> ''L''<sub>Omy</sub> -> ''E''<sub>F</sub>
* CI
* CII
* CI<small>&</small>CII
* CIV
* FAO
* CGPDH




== Orthogonal ==
=== Orthogonal ===


# '''Orthogonal coupling -> substrate control''' (mono- or bidirectional)
# '''Orthogonal coupling -> substrate control''' (mono- or bidirectional)
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== Diametral ==
=== Diametral ===


# '''Diametral coupling -> substrate ->> control''' (mono- or bidirectional)
# '''Diametral coupling -> substrate ->> control''' (mono- or bidirectional)
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= References and Notes =
== References and notes ==


* [[MitoPedia: Respiratory states]]
* [[MitoPedia: Respiratory states]]

Revision as of 03:18, 19 August 2015

This page is in preparation (intern wiki: SUIT protocol library intern)

Substrate-uncoupler-inhibitor titration (SUIT) protocols

Classes of SUIT protocols with coupling and substrate control

Definition of states

Define substrates

  • CI: PM, GM, PGM, M, ..
  • CII: S(Rot), S, SM, ..
  • CI&II: PS, GS, PMS, GMS, PGMS, ..
  • CIV: AsTM
  • FAO: Oct, Pal, ...
  • CGPDH:
  • c

Define coupling states

  • L, P, E

Define residual oxygen consumption

  • ROX: Rot&Ama, Rot&Mna&Ama, Cn, Az, ..


Substrate control states

Single substrate control states

  1. CI
  2. CII
  3. CI&CII
  4. CIV
  5. FAO
  6. CGPDH

Multiple substrate control states

A. CI-CII-CIV

  1. CI + CII
  2. CI + CII + CIV
  3. CI + CI&CII + CII
  4. CI + CI&CII + CII + CIV

B. FAO-CI-CII-CIV

  1. FAO + CI + CII
  2. FAO + CI + CII + CIV
  3. FAO + CI + CI&CII + CII
  4. FAO + CI + CI&CII + CII + CIV

C. CGPDH-CI-CII-CIV

  1. CGPDH + CI
  2. CGPDH + CI + CII
  3. CGPDH + CI + CII + CIV
  4. CGPDH + CI + CI&CII + CII
  5. CGPDH + CI + CI&CII + CII + CIV
  6. CI + CGPDH + ...
  7. CI + CI&CII + CGPDH + ...
  8. CI&CII + CGPDH + ...

D. FAO-CGPDH-CI-CII-CIV

  1. FAO + CGPDH + CI + CII
  2. FAO + CGPDH + CI + CII + CIV
  3. FAO + CGPDH + CI + CI&CII + CII
  4. FAO + CGPDH + CI + CI&CII + CII + CIV
  5. ...


Control patterns

Linear

  1. Linear coupling control: at a single substrate state (classical State 3 -> State 4, or P -> LT).
  2. Linear substrate control: at a single coupling state (unusual).
  3. Monodirectional linear coupling control: LN -> PD -> EF
  4. Bidirectional linear coupling control: LN -> PD -> LOmy -> EF


Orthogonal

  1. Orthogonal coupling -> substrate control (mono- or bidirectional)
  2. Orthogonal substrate -> coupling control (mono- or bidirectional)


Diametral

  1. Diametral coupling -> substrate ->> control (mono- or bidirectional)
  2. Diametral substrate -> coupling ->> control (mono- or bidirectional)


References and notes

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